郑州大学学报（医学版）2023，Vol.58Issue(6)：781-785,5.DOI:10.13705/j.issn.1671-6825.2023.06.058
长链非编码RNA SNHG15对肾细胞癌细胞增殖、凋亡、迁移和侵袭的影响
Effects of LncRNA SNHG15 on proliferation,migration and invasion of RCC cells
摘要
Abstract
Aim:To investigate the effects and mechanism of LncRNA SNHG15 on proliferation,apoptosis,migration,and invasion of renal cell carcinoma(RCC)cells.Method:786-O and ACHN cells were divided into the blank control group transfected with the empty vector pcDNA3.1 and the SNHG15 group transfected with the SNHG15 expression plas-mid;divided into shRNA negative control group transfected with unrelated sequences(shNC group),short hairpin RNA group 1 and group 2 transfected short hairpin RNA targeting SNHG15(shSNHG15#1 group and shSNHG15#2 group).MTT assay,Caspase 3/7 kit,scratch assay,and Transwell chamber were used to detect the proliferation,apoptosis,migration,and invasion ability of RCC cells.The relationship between SNHG15,Myc,and miR-665 was detected through bioinformatics prediction and dual luciferase reporter assay.Results:Overexpression of SNHG15 significantly increased the expression of SNHG15 in RCC cells,while short hairpin RNA transfection inhibited its expression(P<0.001).Overexpression of SNHG15 promoted the proliferation,apoptosis resistance,migration,and invasion abilities of 786-O and ACHN cells(P<0.05),while knocking down SNHG15 inhibited the above malignant biological behavior of RCC cells(P<0.05).The dual luciferase reporter assay confirmed that SNHG15 could directly bind to miRNA-665,thereby relieving the inhibition of miR-665 on its target protein Myc and increasing the expression of Myc protein.Conclusion:SNHG15 may promote the prolifera-tion,apoptosis resistance,migration,and invasion abilities of RCC cells through increasing the expression of Myc protein by competitive adsorption of miR-665.关键词
SNHG15/肾细胞癌/Myc/miR-665/增殖/凋亡/迁移/侵袭Key words
SNHG15/renal cell carcinoma/Myc/miR-665/proliferation/apoptosis/migration/invasion分类
医药卫生引用本文复制引用
张绍谨,闫泽晨,李腾,陈昆仑,刘雨轩,李松超,王静,何娟娟,张卫星..长链非编码RNA SNHG15对肾细胞癌细胞增殖、凋亡、迁移和侵袭的影响[J].郑州大学学报（医学版）,2023,58(6):781-785,5.基金项目
河南省医学科技攻关计划联合共建项目(LHGJ20190298) （LHGJ20190298）
