摘要
Abstract
Aim:To investigate the effects of fucoidan(Fuc)on the hippocampal neurons of neonatal rats induced by oxygen and glucose deprivation(OGD).Methods:The hippocampal neurons of newborn rats were isolated and allocated into Mock group(without treatment),OGD group(with OGT treatment),Fuc group(cultured with 100 μg/mL Fuc for 7 days)and OGD +Fuc group(after OGD,cultured with Fuc).MTT was used to detect cell viability,AnnexinⅤ-FITC/PI staining was used to detect cell apoptosis.RT-qPCR was used to detect the expression of inflammation markers IL-1β,TNF-α and iNOS mRNA.The kit was used to detect the levels of SOD and MDA.Western blot was used to detect the expressions of au-tophagy marker proteins LC3,Beclin-1 and P62,the expressions of apoptosis-related proteins Cleaved Caspase-3 and-9,and PI3K/Akt signaling pathway related proteins PI3K,p-PI3K,Akt and p-Akt.Results:In OGD group,the cell viability de-creased,the apoptosis rate and the expressions of Cleaved Caspase-3,Cleaved Caspase-9 increased;SOD activity increased,and MDA content decreased(P<0.05);the expressions of IL-1β,TNF-α,iNOS mRNA increased(P<0.05);the expres-sions of LC3 and Beclin-1 increased(P<0.05);the expressions of p-PI3K,p-Akt and P62 decreased(P<0.05).Com-pared with OGD group,OGD +Fuc group showed antagonistic effect on the above indexes(P<0.05).Conclusion:Fuc can reduce inflammation,autophagy and oxidative stress by activating the PI3K/Akt signaling pathway,thus protect neurons in-duced by OGD.关键词
岩藻多糖/氧糖剥夺/神经元损伤/新生大鼠Key words
fucoidan/oxygen and glucose deprivation/neuronal damage/neonatal rat分类
医药卫生
