化工进展2023，Vol.42Issue(11)：5891-5899,9.DOI:10.16085/j.issn.1000-6613.2022-2242
沼泽红假单胞菌产类胡萝卜素及抗氧化性
Production and antioxidant activities of carotenoids from Rhodopseudomonas palustris
摘要
Abstract
Carotenoids are widely used as fat-soluble natural pigments and antioxidants.Here we chose Rhodopseudomonas palustris,a typical purple nonsulfur photosynthetic bacteria,to produce carotenoids under photo-anaerobic conditions.The effects of culture conditions such as organic acid types,salt concentration and light intensity on the cell growth,carotenoid content and composition were studied,and the antioxidant activities of carotenoids were also explored.The results showed that lactic acid as carbon source produced the highest biomass and carotenoid content among the eight organic acids tested.The optimum light intensity(500lx)for carotenoid content was lower than that required for the growth of Rhodopseudomonas palustris(2000lx).High salt concentration promoted the synthesis of carotenoids,and the optimum concentration(7.5g/L)was three times that required for its growth.Under optimized conditions,the carotenoid content reached 15.35mg/L.Six kinds of carotenoids were detected,and the composition was greatly affected by the culture conditions.High salt concentration and strong light favored the synthesis of 3,4-didehydrorhodopin,which might be a mechanism for photosynthetic bacteria to adapt to environmental stress.The antioxidant activity test and data analysis showed that antioxidant activities of the produced carotenoids highly depended on their composition,and OH-spirilloxanthin and 3,4-didehydrorhodopin showed strong antioxidant activities.关键词
光合细菌/类胡萝卜素/抗氧化活性/有机酸Key words
photosynthetic bacteria/carotenoid/antioxidant activity/organic acids分类
生物科学引用本文复制引用
李南楠,彭思雨,闫海,尹春华..沼泽红假单胞菌产类胡萝卜素及抗氧化性[J].化工进展,2023,42(11):5891-5899,9.基金项目
国家重点研发计划(2019YFC1906302,2019YFC1906301) （2019YFC1906302,2019YFC1906301）
国家自然科学基金(11971055). （11971055）
