脂磷壁酸激活奶牛乳腺中NUPR1表达及分布研究OA

The aim of this study was to investigate the expression and distribution of NUPR1 gene in dairy cows with mastitis.To establish a lipoteichoic acid-induced inflammation model of dairy cow mammary epithelial cells(MAC-T)in vitro,the distribution of Nucleus Protein 1(NUPR1)gene in the mammary tissue of diseased dairy cows was determined by immunohistochemistry(IHC).qRT-PCR and WB were used to detect the expression of inflammation-related factors,NUPR1 gene and its upstream and downstream genes in the mastitis tissue and mammary epithelial cell inflammation model of dairy cows.The results were as follows:qRT-PCR and WB results showed that IL-1β,IL-6,IL-8 and TNF-α were significantly up-regulated(P＜0.01)in breast pathological tissues and cell inflammation models.IHC results showed that the expression of NUPR1 in the mammary epithelial cells and diseased mammary tissue of dairy cows was significantly(P＜0.05)or extremely significantly(P＜0.01)higher than that in the normal group,DNMT1 was down-regulated,KLF4,SESN2 and SOCS3 genes were up-regulated(P＜0.01)in the pathological mammary tissue and mammary epithelial cell inflammation model of dairy cows with mastitis.The results provide experimental and theoretical basis for further exploring the regulatory mechanism of NUPR1 gene in the pathological process of mastitis.