海洋科学2023，Vol.47Issue(9)：40-52,13.DOI:10.11759/hykx20230518001
SsC1qDC基因在毛蚶免疫防御中的作用
A novel C1q domain-containing gene(SsC1qDC)from Scapharca subcrenata participates in the immune defense against pathogen infection
摘要
Abstract
The C1q domain-containing proteins(C1qDCs)have been demonstrated to play crucial roles in the im-mune responses of invertebrates,with very few reports in the ark shells.In the present study,a C1qDC gene with a typical C1q domain(designated as SsC1qDC)was identified from the ark shell Scapharca subcrenata and its possi-ble immune responses against pathogen infection was also characterized.The full-length cDNA sequence of SsC1qDC was 711 bp,encoding a peptide of 199 amino acids with an N-terminal signal peptide.SsC1qDC was clustered with the C1qDCs from bivalves in the phylogenetic tree.SsC1qDC was widely expressed in the tissues,with the high expression level in hepatopancreas and adductor muscles.The expression of SsC1qDC was increased significantly from eggs to eye-point larvae stage and induced remarkably by Vibro parahaemolyticus infection.Moreover,compared to the controls,knockdown of SsC1qDC resulted in a remarkable increase of mortality and apoptotic levels of ark shells after V.parahaemolyticus challenge,and thus a significant reduction of total number and phagocytosis of hemocytes.Meanwhile,knockdown of SsC1qDC could significantly inhibit the expression of maternally derived immune-related genes(Vg,LSZ,Dscam,TEP and SsC1qDC-3),while the expression of C3,PAF3,SsC1qDC-1 and SsC1qDC-2 was up-regulated remarkably.Taken together,these results suggested that SsC1qDC performs crucial roles in immune defense against pathogen infection in ark shells,which is of great guiding significance to the immunology of shellfish and the breeding of new disease-resistant strains.关键词
SsC1qDC/毛蚶(Scapharca subcrenata)/表达图谱/RNA干扰/免疫防御Key words
SsC1qDC/Scapharca subcrenata/expression profiles/RNA interference/immune defense分类
农业科技引用本文复制引用
彭强,王进京,王春德,卢霞,刘相全,刘桂龙,徐鑫,许贺,宁军号..SsC1qDC基因在毛蚶免疫防御中的作用[J].海洋科学,2023,47(9):40-52,13.基金项目
国家自然科学基金资助项目(31972791) （31972791）
山东省农业良种工程资助项目(2020LZGC016) （2020LZGC016）
山东省烟台市科技项目专项基金资助项目(2022XCZX083) （2022XCZX083）
山东省现代农业产业技术体系建设项目(SDAIT-14)the National Natural Science Foundation of China,No.31972791 （SDAIT-14）
The Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province,No.2020LZGC016 （）
The Scientific and Techno-logical Project of Yantai,Shandong Province,No.2022XCZX083 （）
The Earmarked Fund for Shandong Modern Agro-Industry Technology Re-search System,No.SDAIT-14 （）
