海洋科学2023，Vol.47Issue(9)：53-60,8.DOI:10.11759/hykx20210810002
微塑料对近江牡蛎免疫力的影响
Effect of polystyrene microplastics on oyster immunity
摘要
Abstract
We aimed to study the effect of microplastics on oyster immunity by injecting oysters(average shell length:6.6 cm)with different concentrations of polystyrene microplastics(0.05,0.5,and 5 mg·L-1,with sterilized saline water as diluent)via intrashell injection(without damaging the soft tissue of oyster).The gills were sampled at 24,48,and 72 h,respectively,and the activities of six immune-related indices,including alkaline phosphatase(AKP),superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),nitric oxide synthase(NOS),and acetylcholinesterase(AChE),were determined.After 24 h of treatment,the concentration of microplastics had a significant effect on gill AKP activity in the oysters(P＜0.05).AKP,SOD,and structural NOS initially increased and subsequently decreased.After 48 h of treatment,increasing the microplastic concentration led to a significant decrease in AChE activity(P＜0.05),and CAT,SOD,and AKP initially increased and subsequently decreased.After 72 h of microplastic expo-sure,the activities of AKP and AChE initially decreased and then increased with an increase in microplastic concen-tration,whereas the activities of SOD,CAT,and inducible NOS initially increased and subsequently decreased.The MDA content increased with time and concentration of microplastics.These results show that microplastics induce an immune stress response in oysters,and longer stress time causes a more intense immune stress response.关键词
近江牡蛎/微塑料/酶活力/免疫指标Key words
oyster/microplastics/enzyme activity/immune index分类
农业科技引用本文复制引用
牟红莉,王瑞旋,王俊,林小植,王江勇..微塑料对近江牡蛎免疫力的影响[J].海洋科学,2023,47(9):53-60,8.基金项目
国家自然科学基金项目(31902416) （31902416）
水生细菌的耐药性传递规律及主要机制研究(2022ZDX4029) （2022ZDX4029）
水生弧菌多重耐药性的传递机制研究(2021ZDJS041)National Natural Science Foundation Program,No.31902416 （2021ZDJS041）
Research Project on Drug Resistance Transmission Patterns and Main Mechanisms of Aquatic Bacteria,No.2022ZDX4029 （）
Research Project on the Transmission Mechanism of Multiple Drug Resistance in Aquatic Vibrio,No.2021ZDJS041 （）
