海洋科学2023，Vol.47Issue(9)：71-80,10.DOI:10.11759/hykx20221124002
LED光谱对许氏平鲉视网膜形态的影响
Effect of the LED spectrum on retinal morphology of Sebastes schlegelii
摘要
Abstract
Light is a complex environmental factor that significantly affects the survival,feeding,growth,and vis-ual perception of fish.To investigate the effects of Light Emitting Diode(LED)spectra on the retinal structure and the number and thickness of the cells in each retinal layer of Sebastods schlegelii,1,000 S.schlegelii juveniles(body mass 38.80±0.43 g,body length 10.20±0.17 cm)were cultured in five spectral treatment groups:blue light(λ450 nm),green light(λ525 nm),yellow light(λ590 nm),white light(λ460 nm),red light(λ630 nm),green light(λ525 nm),yellow light(λ590 nm),white light(λ460 nm),and red light(λ630 nm).The white light group was the control group,and the other four groups were the treatment groups.After 60 days of treatment,samples were taken from the retinas of each group for observation.The results showed that the different LED spectra did not affect the retinal structure of S.schlegelii,but the number of retinal cells noted after treatment under each spectrum was slightly different.Under the five different spectral conditions,the number of cells in the inner retinal layer in the blue light group(24.33±2.08)was significantly lower per unit area(100 µm×100 µm)than that in the white light group(P＜0.05);the number of cells in the outer nuclear layer was significantly lower in the red light group(33.33±3.51)than that in all of the other groups except the white light group(P＜0.05).The number of cells in the outer nuclear layer of the red light group(33.33±3.51)was significantly lower than that in all of the other groups except the white light group(P＜0.05).A comparison of the number of cells in each retinal layer of each spectrum showed that the blue light group had the lowest number along with the red light group,and the differences between the other layers were not significant.In summary,the retinal thickness and cell number of S.schlegelii juveniles maintained in different spectral treatment groups underwent adaptive changes in response to changes in the external light environment.This study provides a theoretical basis for revealing the adaptive changes of S.schlegelii to a lighted environment and provides a theoretical reference for setting the lighting conditions for culturing S.schlegelii juveniles.关键词
许氏平鲉(Sebastods schlegelii)/LED光谱/视网膜结构/视网膜厚度/视网膜细胞数目Key words
Sebastes schlegelii/LED spectrum/retinal strcture/retinal thickness/number of retinal cells分类
农业科技引用本文复制引用
吴燕玲,孙飞,蔡皓玮,李鑫,刘鹰,刘松涛,马贺..LED光谱对许氏平鲉视网膜形态的影响[J].海洋科学,2023,47(9):71-80,10.基金项目
国家自然科学基金项目(32202961) （32202961）
辽宁省科学技术计划资助项目(2021JH2/10200011) （2021JH2/10200011）
教育部重点实验室开放课题资助项目(202202) （202202）
大连市领军人才资助项目(2019RD12) （2019RD12）
国家贝类产业技术体系岗位科学家项目(CARS-49)National Natural Science Foundation of China,No.32202961 （CARS-49）
Liaoning Province Science and Technology Plan Project,No.2021JH2/10200011 （）
Key Laboratory Open Subject of Environment Controlled Aqua-culture,No.202202 （）
Innovation Support Program for High-level Talents of Dalian City,No.2019RD12 （）
Modern Agro-industry Technology Research System,No.CARS-49 （）
