净水技术2023，Vol.42Issue(z2)：141-146,6.DOI:10.15890/j.cnki.jsjs.2023.s2.024
饮用水中产气荚膜梭状芽孢杆菌滤膜法检测的优化
Optimization of Filtration Membrane Method for Clostridium perfringens in Drinking Water
曹新垲 1张琦 1尹宝国 1张宝华 1张明明1
作者信息
- 1. 北京市自来水集团有限责任公司水质监测中心,北京 100192
- 折叠
摘要
Abstract
Clostridium perfringensare widely existing pathogenic bacteria in natural environment.It is a routine test item in National Standard for Food Safety Drinking Natural Mineral Water(GB 8538-2016).With the increasing attention to the microbial safety of drinking water,the detection method of Clostridium perfringens had been added to the recently released Standards Examination Methodsfor Drinking Water——Part 12:Microbiological Indices(GB/T 5750.12-2023).In this paper,the testing method was optimized from the aspects of consumables and testing conditions.The results showed that the culture medium,filter material,pore size and film sticking method had important effects on the detection results.The conclusion was that the finished medium was used and the disposable mixed cellulose filter membrane(0.22 µm),reverse attached to the culture medium and cultured at 36～37 ℃can improve the accuracy of detection.关键词
饮用水/产气荚膜梭状芽孢杆菌/滤膜法/优化/检测方法Key words
drinking water/Clostridium perfringens/filtration membrane method/optimization/test method分类
医药卫生引用本文复制引用
曹新垲,张琦,尹宝国,张宝华,张明明..饮用水中产气荚膜梭状芽孢杆菌滤膜法检测的优化[J].净水技术,2023,42(z2):141-146,6.
