净水技术2023，Vol.42Issue(z2)：147-151,5.DOI:10.15890/j.cnki.jsjs.2023.s2.025
酶底物法检测水中菌落总数培养24h和48h的比较试验
Comparative Test of Total Number of Bacterial Colonies in Water Cultured for 24 h and 48 h by Enzyme Substrate Method
翁国永 1王海荣 2项兆邦 3周正4
作者信息
- 1. 浙江省城市供水水质监测网台州监测站,浙江台州 318020||台州市黄岩城乡自来水有限公司,浙江台州 318020
- 2. 台州自来水公司,浙江台州 318000
- 3. 浙江泰诚环境科技有限公司,浙江台州 318000
- 4. 爱德士缅因生物制品贸易<上海>有限公司,上海 200336
- 折叠
摘要
Abstract
In 2023,the composite enzyme substrate method is included in the Standard Examination Methods for Drinking Water——Part 12:Microbial Indeices(GB/T 5750.12-2023)as a new method for detecting the total number of bacterial colonies in water.In order to facilitate more scientific and standard use of complex enzyme substrate method to detect the total number of colonies in drinking water,this paper adopted complex enzyme substrate method to detect 80 groups of actual samples of drinking water and source water,and analyzed the detection results of two different culture times of 24 h and 48 h.The test results showed that there was a significant difference between the 24 h culture results of SimPlate method and 48 h culture results.The paired t-test analysis showed that the 24 h culture results of enzyme substrate method and 48 h culture results P=0.000(＜0.05),which had a significant statistical difference,It was found that some pathogenic bacteria could not be detected by SimPlate culture for 24 h.Therefore,when enzyme substrate method was used to detect the total number of colonies in drinking water and source water,the culture time should be at least 48 hours to meet the detection requirements of the laboratory on the total number of colonies in drinking water and source water.关键词
酶底物法/培养时间/菌落总数/生活饮用水/菌种鉴定Key words
enzyme substrate method/incubation time/total number bacterial colonies/domestic drinking water/species identification分类
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翁国永,王海荣,项兆邦,周正..酶底物法检测水中菌落总数培养24h和48h的比较试验[J].净水技术,2023,42(z2):147-151,5.
