实用临床医药杂志2023，Vol.27Issue(22)：55-61,66,8.DOI:10.7619/jcmp.20232362
红景天苷对1-甲基-4-苯基-1-2-3-6-四氢吡啶诱导帕金森病小鼠的作用及机制
Effect and mechanism of salidroside for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in mice
摘要
Abstract
Objective To investigate the effect and mechanism of salidroside on1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease(PD)in mice.Methods C57BL/6J mice were induced by MPTP to establish a PD mouse model and the movement track of mice was re-corded by open field experiment.The expression of tyrosine hydroxylase(TH),α-synuclein(α-syn),nuclear factor E2 associated factor 2(Nrf2)and quinone oxidoreductase 1(NQO1)were detected by immunohistochemistry in brain tissue of mice.SN4741 cells were stimulated by MPTP to establish a PD cell model in vitro.MPTP stimulation of SN4741 cells to establish an in vitro cell mod-el of PD.After pretreatment with salidroside,TUNEL was used to detect cell apoptosis in each group.The expressions of TH,α-syn,Nrf2 and NQO1 were detected by immunofluorescence.Nrf2 expres-sion was knocked down by being transfected with si-RNA,and apoptosis condition was detected by TUNEL assay.The expression levels of interleukin-1β(IL-1β)and interleukin-18(IL-18)protein in brain tissue extract and cell culture were detected by enzyme-linked immunosorbent assay(ELISA).Results The open field experiment results showed that salidroside could improve 3 min total distance of activity,average speed and activity track of PD mice(P＜0.05).Compared with the PD model mice,the salidroside treated mice showed significantly increased expression of TH,Nrf2 and NQO1 in the brain,and reduced expression of IL-18,α-Syn,IL-1β.Compared with the MPTP stimulated cells,the salidroside pretreatment group showed a decrease in cell apoptosis and an increase in the expressions of TH,Nrf2 and NQO1,but reduced expressions in α-Syn,IL-1β and IL-18.After si-RNA knockdown of Nrf2 expression,the protective effect of salidroside on MPTP stimulated cells weakened or even disappeared.Conclusion Salidroside may alleviate neuronal apoptosis by activating Nrf2-ARE signaling pathway,which is expected to be a new drug for PD treatment.关键词
红景天苷/核因子E2相关因子2/抗氧化反应元件/帕金森病/细胞凋亡Key words
salidroside/nuclear factor E2 associated factor 2/antioxidant reaction element/Parkinson's disease/apoptosis分类
医药卫生引用本文复制引用
孙梓旭,董霞,赵宝民,贾海丽,马晓瑞,谷伟..红景天苷对1-甲基-4-苯基-1-2-3-6-四氢吡啶诱导帕金森病小鼠的作用及机制[J].实用临床医药杂志,2023,27(22):55-61,66,8.基金项目
河北省医学科学研究课题基金资助项目(20220622) （20220622）
