实用临床医药杂志2023，Vol.27Issue(22)：67-70,76,5.DOI:10.7619/jcmp.20232167
刺五加精制多糖对尼古丁诱导的学习记忆障碍小鼠的影响
Effects of acanthopanax refined polysaccharide in mice with nicotine-induced learning and memory impairment
邹婷 1刘友昊 2高晓岩 1邓代千 1吴宜艳1
作者信息
- 1. 牡丹江医学院,黑龙江牡丹江,157011
- 2. 牡丹江医学院附属红旗医院,黑龙江牡丹江,157011
- 折叠
摘要
Abstract
Objective To observe the effect of acanthopanax refined polysaccharide(ASPS)on nicotine-induced learning and memory impairment in mice.Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments:the Morris and the new object recognition batch.Each batch was randomly divided into 6 groups ac-cording to body weight:blank control group,model group,drug positive group,high-dose ASPS group,medium-dose ASPS group,and low-dose ASPS group.Except for the blank group,the remai-ning 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model.After 24 hours of injection of nicotine at the 7th day,the drug posi-tive group was gavaged piracetam for 800 mg/kg,and the high-dose,medium-dose and low-dose ASPS groups were gavaged for 270,90 and 30 mg/kg of ASPS respectively for 7 days.The learning and memory ability of mice was detected by water maze test and new object recognition test,respec-tively.After the two tests,superoxide dismutase(SOD)activity in serum and 5-Hydroxytryptamine(5-HT)content in hippocampal tissue were detected.Results The results of new object recognition experiment showed that the discrimination indexes of the high-,medium-and low-dose ASPS groups were significantly higher than that of the model group(P＜0.01 or P＜0.05).The results of water maze experiment showed that the time to find the platform in the spatial search experiment was signifi-cantly shorter in the high-and medium-dose ASPS groups than that in the model group(P＜0.05).In the positioning voyage test,the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group(P＜0.05);the proportions of Ⅲ quadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group(P＜0.05).The high-,medium-and low-dose ASPS groups were significantly higher than those in the model group(P＜0.01);the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high-and medium-dose ASPS groups was significantly higher than that in the model group(P＜0.01 or P＜0.05).Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice,relieve the damage of hippocampal neurotransmitters,and regulate oxidative stress in vivo.The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.关键词
刺五加精制多糖/学习记忆/抗氧化作用/神经递质/超氧化物歧化酶/五羟色胺Key words
eleuthero refined polysaccharides/learning and memory/antioxidant effect/neurotransmitter/superoxide dismutase/5-Hydroxytryptamine分类
医药卫生引用本文复制引用
邹婷,刘友昊,高晓岩,邓代千,吴宜艳..刺五加精制多糖对尼古丁诱导的学习记忆障碍小鼠的影响[J].实用临床医药杂志,2023,27(22):67-70,76,5.
