马唐生防真菌MA4的分离鉴定及其生物学特性OA

[Objective]To screen biocontrol fungi of Digitaria sanguinalis and explore the potential of its application in weed biological control.[Method]The biocontrol strains with strong pathogenicity to Digitaria sanguinalis were isolated from naturally diseased plants by tissue isolation method,and their taxonomic status was determined by morphological observation,sequence homology comparison and phylogenetic analysis of ITS,β-Tubulin and EF-1α gene.Biological characteristics of the biocontrol fungi was determined by single factor test.Infection effect on several common weeds and safety to common crops were also determined by whole plant bioassay.[Result]The isolated Digitaria sanguinalis biocontrol fungi named MA4 was identified as Fusarium fujikuroi.The most suitable media for MA4 growth were PDA and PSA.The most suitable culture conditions for MA4 growth and spore production were temperature of 28-30℃,pH of 6.0-8.0 and dark culture.Fusarium fujikuroi MA4 had good control effect on Digitaria sanguinalis,the fresh weight control effect was 91.4%,and the fresh weight inhibition rates of Alopecurus aequalis,Setaria viridis and Aster subulatus were 63.8%,80.6%and 69.4%,respectively.Fusarium fujikuroi MA4 is relatively safe to dicotyledonous crops.[Conclusion]Fusarium fujikuroi MA4 strain has good control effect on grasses such as Digitaria sanguinalis and is safe to dicot crops,and it has great potential to be developed into a biological herbicide.