生物灾害科学2023，Vol.46Issue(4)：425-431,7.DOI:10.3969/j.issn.2095-3704.2023.04.64
TasA抑菌蛋白对烟草黑胫病的防治效果研究
Biocontrol Effects of Antifungal Protein TasA on Tobacco Black Shank
摘要
Abstract
[Objective]Tobacco black shank is a soil-borne disease caused by fugal pathogens of Phytophthora nicotianae,typically infecting the roots and stems of tobacco plants and causing serious economic losses to the global tobacco industries.Therefore,recombinant strains overexpressing TasA were constructed to reveal the inhibitory effect of TasA on tobacco black shank.Moreover,the heterologous expression level of TasA was altered to improve its inhibitory effects.[Method]The TasA gene of B.subtilis was PCR amplified and overexpressed in Escherichia coli BL21(DE3)strain.The inhibitory effect of TasA protein on tobacco black shank was studied by using P.nicotianae as a pathogen indicator.In addition,the inhibitory effect of TasA under different expression levels was also studied by cloning TasA gene into three vectors with different copy numbers.[Result]Three recombinant E.coli strains(pACYC-TasA,pCDF-TasA,and pT7473-TasA strain)were successfully constructed.SDS-PAGE results revealed that TasA protein was overexpressed in all recombinant strains and its molecular weight was around 35 ku.The mycelial inhibition rate of P.nicotianae in pACYC-TasA,pCDF-TasA,and pT7473-TasA strain was 14.85%,25.55%,and 13.45%,respectively.[Conclusion]TasA protein shows a significant inhibitory effect on tobacco black shank,and recombinant strains overexpressing recombinant plasmid of pCDF-TasA exhibit the best inhibitory effect on tobacco black shank.关键词
烟草黑胫病/烟草疫霉菌/TasA蛋白/PCR扩增/异源表达Key words
tobacco black shank/Phytophthora nicotianae/TasA protein/PCR amplification/heterologous expression分类
农业科技引用本文复制引用
胡丽榕,王亚明,王倩,宋惠,黄子豪,黄林,钟敏,彭帅英..TasA抑菌蛋白对烟草黑胫病的防治效果研究[J].生物灾害科学,2023,46(4):425-431,7.基金项目
江西省教育厅科技计划项目(GJJ180238) （GJJ180238）
