生物灾害科学2023，Vol.46Issue(4)：469-477,9.DOI:10.3969/j.issn.2095-3704.2023.04.71
南昌市动物园圈养动物3种肠道原虫的分子检测及基因型鉴定
Molecular Detection and Genotyping of Three Enteric Protozoa in Captive Animals from Nanchang Zoo
摘要
Abstract
[Objective]Cryptosporidium,Enterocytozoon bieneusi and Blastocystis sp.are three common parasites,which inhabit in the intestines of humans,domestic animals,and wildlife,primarily causing diarrhea in human and animals.This experiment aimed to investigate the epidemiological status and identify their genotypes of three parasites among captive animals in Nanchang Zoo,and provide references for the prevention and treatment of intestinal parasitic diseases in wild animals.[Method]A total of 42 fecal samples were collected from captive animals including non-human primates,ungulates,perissodactyls,elephants,marsupials,birds,and carnivores in Nanchang Zoo.Total DNA was extracted from the fecal samples,and a PCR assay was employed to amplify the small subunit ribosomal RNA(SSU rRNA)gene of Cryptosporidium,the internal transcribed spacer(ITS)region of E.bieneusi,and the SSU rRNA gene of Blastocystis sp..Positive amplicons were sequenced,aligned,and subjected to phylogenetic analysis to determine the infection status and genotypes/subtypes of these three intestinal parasites.[Result]The results indicated that 17 out of 42 fecal samples were positive by PCR,with a total infection rate of 40.5%(17/42).The infection rates of Cryptosporidium,E.bieneusi,and Blastocystis sp.were 2.4%(1/42),14.3%(6/42),and 23.8%(10/42),respectively.Among the detected animals,the positivity rates were as follows:non-human primates 64.7%(11/17),ungulates 23.5%(4/17),perissodactyls 5.9%(1/17),and marsupials 5.9%(1/17).Furthermore,mixed infections of E.bieneusi and Blastocystis sp.were observed in non-human primates.Through sequence alignment and phylogenetic analysis,one Cryptosporidium genotype(Cryptosporidium sp.apodemus genotype Ⅱ)was identified in ponies,and two E.bieneusi genotypes(D,ALP1)were identified in gibbons,golden snub-nosed monkeys,rhesus monkeys,red-tailed langurs,and alpacas.Five Blastocystis sp.subtypes(ST1,ST2,ST5,ST13,and ST14)were identified in squirrel monkeys,black-and-white colobus monkeys,rhesus monkeys,black leaf monkeys,golden snub-nosed monkeys,antelopes,and kangaroos.Notably,the genotypes ST1,ST5,D,and ALP1 are known as zoonotic genotypes.[Conclusion]Captive animals in Nanchang Zoo were infected with Cryptosporidium,E.bieneusi and Blastocystis sp..The zoonotic genotypes are identified from E.bieneusi and Blastocystis sp.,which have a potential transmission risk.This underscores the need for enhanced monitoring of intestinal protozoa in captive animals,and the implementation of deworming,disinfection,and preventive measures against zoonotic transmission within the zoo setting.关键词
圈养动物/毕氏肠微孢子虫/芽囊原虫/隐孢子虫/分子检测/基因型Key words
captive animal/Enterocytozoon bieneusi/Blastocystis sp./Cryptosporidum spp./molecular detection/genotype分类
农业科技引用本文复制引用
梁玮珈,陈小庆..南昌市动物园圈养动物3种肠道原虫的分子检测及基因型鉴定[J].生物灾害科学,2023,46(4):469-477,9.基金项目
国家自然科学基金项目(32202839)和江西省教育厅科技创新项目(GJJ180185) （32202839）
