解放军医学院学报2023，Vol.44Issue(9)：1025-1029,1059,6.DOI:10.12435/j.issn.2095-5227.2023.016
西妥昔单抗对A-431细胞系增殖和凋亡的影响
Effects of cetuximab on proliferation and apoptosis of A-431 cell line
摘要
Abstract
Background Cutaneous squamous cell carcinoma(cSCC),is one of the most common skin malignant tumors in clinic,accounting for 20%of all skin cancer deaths.Some advanced cSCC patients cannot be effectively treated by operation.The guidelines recommend molecular targeted therapy,immunotherapy,chemotherapy and other systemic therapies to improve prognosis for these patients.Objective To study the effect of cetuximab on the proliferation and apoptosis of cutaneous squamous cell carcinoma cell line A-431 with high expression of epidermal growth factor receptor(epidermal growth factor receptor,EGFR).Methods The expression of EGFR in A-431 cells were screened from the human protein map database and verified by Western blot experiment.A-431 cells were randomly divided into control group and cetuximab treatment group(200µG/mL),and MTT assay was used to detect the effect of cetuximab on the proliferation and toxicity of A-431 cells in vitro.Flow cytometry was performed to detect apoptosis.Results In the human protein map database,41 kinds of normal tissues and tumor cell lines were compared,among which the expression of EGFR in A-431 cells was the highest.The expression of EGFR in A-431 cell was significantly higher than that in human immortalized epidermal cell HaCat by Western blot.MTT assay showed that cetuximab strongly inhibited the proliferation of A-431 cells at the concentration of 200µg/mL and killed about 61.35%of tumor cells(P<0.01).Flow cytometry showed that the apoptosis rate of cetuximab at the concentration of 150µg/mL was 29.85%,while that of the control group was only 10.44%(P<0.01).Conclusion Cetuximab has good killing ability to cutaneous squamous cell carcinoma cell line A-431 with high expression of EGFR and significantly increases the apoptosis of tumor cells,which provides a therapeutic strategy for cSCC patients who cannot be treated by surgery.关键词
皮肤鳞状细胞癌/表皮生长因子受体/西妥昔单抗/细胞增殖/凋亡Key words
cutaneous squamous cell carcinoma/epidermal growth factor receptor/cetuximab/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
殷祥烨,姜伟乾,韩愚弟,韩岩..西妥昔单抗对A-431细胞系增殖和凋亡的影响[J].解放军医学院学报,2023,44(9):1025-1029,1059,6.基金项目
国家自然科学基金青年项目(82202473) （82202473）
