青岛大学学报（医学版）2023，Vol.59Issue(5)：633-638,6.DOI:10.11712/jms.2096-5532.2023.59.144
白藜芦醇对ox-LDL诱导的内皮细胞铁死亡影响
INFLUENCE OF RESVERATROL ON OX-LDL-INDUCED FERROPTOSIS IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
摘要
Abstract
Objective To investigate the influence of resveratrol on oxidized low-density lipoprotein(ox-LDL)-induced ferroptosis in human umbilical vein endothelial cells(HUVECs).Methods The ferroptosis in HUVECs was induced by ox-LDL.In this study,control group,ox-LDL group,and low-,middle-,and high-dose resveratrol groups were set.Cell viability and levels of malondialdehyde(MDA),glutathione(GSH),ferrous ion(Fe2+),and reactive oxygen species(ROS)in cells were measured.Western blot and immunofluorescence method were applied to measure the protein expression of glutathione peroxidase 4(GPX4),solute carrier family 7 member 11(SLC7A11),and ferritin heavy chain 1(FTH1)in cells.Results Compared with the control group,the ox-LDL group had significantly decreased cell viability and expression levels of GSH,GPX4,SLC7A11,and FTH1,and significantly increased MDA,Fe2+,and ROS levels.Compared with the ox-LDL group,the low-,middle-,and high-dose resveratrol groups had significantly increased cell viability and expression levels of GSH,GPX4,SLC7A11,and FTH1,and significantly decreased MDA,Fe2+,and ROS levels(F=9.93-722.16,P＜0.05).Conclusion Resveratrol may protect HUVECs from ferroptosis by activating the SLC7A11/GPX4 signaling pathway.关键词
白藜芦醇/人脐静脉内皮细胞/铁死亡Key words
resveratrol/human umbilical vein endothelial cells/ferroptosis分类
医药卫生引用本文复制引用
游福蓉,杜占慧,宗丽娟,王勤拯,张成,邢泉生..白藜芦醇对ox-LDL诱导的内皮细胞铁死亡影响[J].青岛大学学报（医学版）,2023,59(5):633-638,6.基金项目
国家自然科学基金资助项目(82071583) （82071583）
