青岛大学学报（医学版）2023，Vol.59Issue(5)：639-644,6.DOI:10.11712/jms.2096-5532.2023.59.152
广东和山东地区鼻咽癌组织EB病毒BART miRNA cluster 1多态性比较
POLYMORPHISM OF EPSTEIN-BARR VIRUS BART MIRNA CLUSTER 1 IN NASOPHARYNGEAL CARCINOMA TISSUES FROM GUANGDONG AND SHANDONG,CHINA
摘要
Abstract
Objective To determine the polymorphism of Epstein-Barr virus(EBV)BamH Ⅰ A rightward transcript(BART)microRNA(miRNA)cluster 1 gene in patients with nasopharyngeal carcinoma(NPC)and healthy donors from Guang-dong and Shandong,China,and to investigate its association with the occurrence of NPC.Methods Nested polymerase chain reaction and DNA sequencing were used to detect the sequences of BART miRNA cluster 1 gene in samples from 183 patients with NPC(79 patients from Guangdong and 104 patients from Shandong)and 113 healthy donors(59 donors from Guangdong and 54 donors from Shandong).Lasergene and MEGA were used to perform comparison and phylogenetic tree analysis for the sequences,and the variations were typed according to the signature changes and phylogenetic tree.The distributions of BART miRNA cluster 1 gene variants/subtypes were compared between different populations and different areas.Results Six variants/subtypes,namely BART-A,-B1,-B2,-C,-D1,and-D2,were identified in BART miRNA cluster 1 gene sequences from 296 samples.Among them,BART-D2 and-D1 were the main subtypes.In Guangdong,BART-D2 was most common,while in Shandong,BART-D1 was most common.The detection rates of BART-D2 in patients with NPC and healthy donors in Guangdong were 86.1％(68/79)and 44.1％(26/59),respectively,which were significantly higher than those in patients with NPC(27.9％,29/104)and healthy donors(1.9％,1/54)in Shandong(X2=61.032,27.632;P＜0.001).More importantly,BART-D2 was more prevalent in NPC cases than in healthy donors in both areas(X2=27.444,15.660;P＜0.001).Conclusion The variations of EBV BART miRNA cluster 1 gene have differences between Guangdong and Shandong.BART-D2 subtype may be mainly prevalent in Guang-dong and related to the occurrence of NPC.关键词
鼻咽癌/疱疹病毒4型,人/BamHⅠ A右向转录物/微RNAs/多态性,单核苷酸Key words
nasopharyngeal carcinom/herpesvirus 4,human/BamH Ⅰ A rightwardtranscripts/microRNAs/polymor-phism,single nucleotide分类
医药卫生引用本文复制引用
高龙峰,王巧祎,张增霞,王云..广东和山东地区鼻咽癌组织EB病毒BART miRNA cluster 1多态性比较[J].青岛大学学报（医学版）,2023,59(5):639-644,6.基金项目
国家自然科学基金资助项目(81171571) （81171571）
