青岛大学学报（医学版）2023，Vol.59Issue(5)：645-650,6.DOI:10.11712/jms.2096-5532.2023.59.163
METTL5对肺腺癌细胞迁移和侵袭的影响及机制
ROLE OF METTL5 IN MIGRATION AND INVASION OF LUNG ADENOCARCINOMA CELLS AND MECHANISMS
摘要
Abstract
Objective To investigate the role of the methyltransferase-like protein 5(METTL5)gene in the migration and invasion of lung adenocarcinoma cells and the potential mechanisms.Methods METTL5 expression levels in lung adenocar-cinoma and paracancerous tissues were compared through the Gene Expression Omnibus(GEO)database.METTL5 protein ex-pression levels in normal lung epithelial cells(BEAS-2B)and lung adenocarcinoma cell lines(A549 and H1975)were determined by Western blot.A549 and H1975 cell lines were infected with the LV3-METTL5-shRNA lentivirus to knockdown the expression of the METTL5 gene,which was verified by Western blot for the silencing effects.After successfully knocking down the METTL5 gene,transwell assay was used to determine the migration and invasion abilities of the two cell lines.The expression of epithelial-mesenchymal transition markers(E-Cadherin and Vimentin proteins)was measured by Western blot.Results The GEO analy-sis showed that the expression of METTL5 was significantly higher in lung adenocarcinoma tissues than in paracancerous tissues,and patients with high METTL5 expression had a significantly poorer prognosis.Lung adenocarcinoma A549 and H1975 cell lines had significantly higher METTL5 levels than the normal lung epithelial cell line BEAS-2B.After knocking down METTL5,A549 and H1975 cells showed significantly reduced migration and invasion abilities,significantly down-regulated expression of the mesen-chymal marker Vimentin,and significantly up-regulated expression of the epithelial marker E-Cadherin.Conclusion METTL5 can increase the migration and invasion of lung adenocarcinoma cells by promoting the process of epithelial-mesenchymal transition.关键词
肺腺癌/蛋白甲基转移酶类/上皮-间质转化/细胞运动Key words
adenocarcinoma of lung/protein methyltransferases/epithelial-mesenchymal transition/cell movement分类
医药卫生引用本文复制引用
彭修法,王蕊红,王晔,刘凤娟,张春玲..METTL5对肺腺癌细胞迁移和侵袭的影响及机制[J].青岛大学学报（医学版）,2023,59(5):645-650,6.基金项目
国家自然科学基金资助项目(82072927) （82072927）
