青岛大学学报（医学版）2023，Vol.59Issue(5)：658-662,5.DOI:10.11712/jms.2096-5532.2023.59.148
HFPO-DA染毒对孕鼠甲状腺内分泌功能的影响
EFFECTS OF EXPOSURE TO HEXAFLUOROPROPYLENE OXIDE DIMER ACID ON THYROID ENDOCRINE FUNCTION IN PREGNANT RATS
摘要
Abstract
Objective To investigate the effects of exposure to hexafluoropropylene oxide dimer acid(HFPO-DA)on thyroid endocrine function in pregnant rats.Methods Sprague-Dawley(SD)rats(pregnant for 0.5 d)were randomly divided in-to control group(deionized water)and low-dose(1 mg/kg),medium-dose(10 mg/kg),and high-dose(100 mg/kg)HFPO-DA groups,with eight rats in each group.The rats were exposed to HFPO-DA daily based on body weight during the gestational period of 0.5 to 19.5 d.On day 19.5,all the pregnant rats were decapitated after anesthesia with chloral hydrate at 100 g/L to measure the growth and development data of the fetal rats.Thyroid tissue morphology was observed with hematoxylin-eosin staining.The se-rum levels of thyroid stimulating hormone(TSH),total thyroxine(TT4),and total triiodothyronine(TT3)in the pregnant rats were measured by enzyme-linked immunosorbent assay.Serum HFPO-DA levels were determined by high performance liquid chro-matography tandem mass spectrometry.Results Compared with the control group,exposure to high-dose HFPO-DA signifi-cantly decreased fetal rats'body weight,body length,and tail length at birth,and significantly increased the levels of TSH and TT3 in pregnant rats(t=5.07-38.64,P＜0.05),with follicular hyperplasia present in the thyroid tissue.Conclusion High-dose HFPO-DA exposure can damage the thyroid structure and disrupt the thyroid hormones of pregnant rats,leading to intraute-rine growth and development retardation of offspring.关键词
六氟环氧丙烷二聚体羧酸/甲状腺激素类/妊娠,动物/胚胎发育Key words
hexafluoropropylene oxide dimer acid/thyroid hormone/pregnancy,animal/embryonic development分类
医药卫生引用本文复制引用
吕娣,谭桂玲,陈国威,刘文东,韩文超..HFPO-DA染毒对孕鼠甲状腺内分泌功能的影响[J].青岛大学学报（医学版）,2023,59(5):658-662,5.基金项目
国家自然科学基金青年科学基金项目(81903271) （81903271）
