青岛大学学报（医学版）2023，Vol.59Issue(5)：698-702,5.DOI:10.11712/jms.2096-5532.2023.59.149
丹参-葛根提取物对小鼠动脉粥样硬化的保护作用
PROTECTIVE EFFECTS OF SALVIA MILTIORRHIZA BUNGE-RADIX PUERARIAE EXTRACT IN MICE WITH ATHEROSCLE-ROSIS
摘要
Abstract
Objective To investigate the protective effects of Salvia miltiorrhiza Bunge-Radix Puerariae extract(DG)in mice with atherosclerosis(AS).Methods Twenty-four ApoE-/-mice(6 weeks old,male)were randomly divided into blank group,model group,low-dose DG group(200 mg/kg),and high-dose DG group(400 mg/kg),with six mice in each group.The mice in the blank group were fed a normal diet,while those in the model group and DG treatment groups were fed a high-sugar and high-fat diet.The blank and model groups were intragastrically given normal saline,while the DG groups received intragastric injec-tion of DG,for a continuous period of 16 weeks.The serum levels of lipids(triglyceride(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C))and oxidative stress factors(malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione(GSH))were measured using biochemical kits.Serum inflammatory factors(tumor necrosis factor-α(TNF-α),interleukin-12(IL-12),soluble intercellular adhesion molecule-1(sICAM-1),and soluble vascular cell adhesion molecule-1(sVCAM-1))were measured by enzyme-linked immunosorbent assay(ELISA).The pathological changes of the aorta were ob-served with oil red O staining and Masson staining.Results Compared with those in the blank group,serum TG,TC,and LDL-C levels in the model group were significantly increased;DG treatment significantly ameliorated dyslipidemia in a dose-de-pendent manner(F=180.0-564.6,P＜0.001).The model group showed a significantly increased serum MDA level and signifi-cantly decreased serum SOD and GSH levels;DG treatment significantly reduced serum MDA levels and increased SOD and GSH levels(F=133.0-194.7,P＜0.001).The inflammatory factors TNF-α,IL-12,sICAM-1,and sVCAM-1 were significantly in-creased in the model group than in the normal mice;and these indicators were significantly inhibited by DG treatment in a dose-de-pendent manner(F=247.0-371.0,P＜0.001).Oil red O staining and Masson staining results showed that compared with the model group,the low-dose and high-dose DG groups had reduced arterial plaque areas and increased collagen fiber content in plaques.Con-clusion DG can reduce lipid peroxidation,inhibit inflammatory response,reduce plaque area,and improve the stability of vulnera-ble plaques in mice with AS,suppressing the development and progression of AS.关键词
丹参-葛根提取物/动脉粥样硬化/氧化性应激/炎症Key words
Salvia miltiorrhiza-Radix Puerariae extract/atherosclerosis/oxidative stress/inflammation分类
医药卫生引用本文复制引用
张咪,张梦瑶,韩彦弢,赵文文..丹参-葛根提取物对小鼠动脉粥样硬化的保护作用[J].青岛大学学报（医学版）,2023,59(5):698-702,5.基金项目
山东省中医药科技项目(2021M137) （2021M137）
