青岛大学学报（医学版）2023，Vol.59Issue(5)：703-708,6.DOI:10.11712/jms.2096-5532.2023.59.168
血卟啉衍生物在3种肺癌细胞系中的细胞摄取和定位
UPTAKE AND LOCALIZATION OF HEMATOPORPHYRIN DERIVATIVES IN THREE LUNG CANCER CELL LINES
摘要
Abstract
Objective To compare the differences in cellular uptake and localization of hematoporphyrin derivatives(HPDs)between different lung cancer cell lines(lung adenocarcinoma A549,lung squamous cell carcinoma H520,and lung small cell carcinoma H446)using the bronchial epithelial cell line BEAS-2B as control.Methods The standard curve of HPD fluores-cence values was plotted using values measured at different concentrations of HPDs.The four cell lines were incubated with HPDs for different time periods and then measured for fluorescence intensity using a multifunctional microplate meter.Flow cytometry was used to quantify the cell uptake and compare the differences in HPD uptake between the four cell lines.The uptake and distri-bution of HPDs in the cells were observed and compared using a laser scanning confocal microscope.Results The uptake of HPDs in the four cell lines increased over time,with some differences in the accumulation rate of HPDs.After cells were incubated with 5 mg/L HPD for 24 h and 48 h,the mean fluorescence intensities of the four cell lines were significantly different(F=199.00,71.15,P＜0.001),with the mean fluorescence intensity significantly higher in A549 cells than in H446,BEAS-2B,and H520 cells,and the mean fluorescence intensity significantly higher in H446 and BEAS-2B cells than in H520 cells(P＜0.05).The four cell lines showed varying intensities of red fluorescent signals distributed in a dot pattern in the cytoplasm when the cells were incubated with 15 mg/L HPDs for 48 h.Conclusion There are differences in HPD uptake between different lung cancer cell lines,with the highest uptake in A549 cells,followed by BEAS-2B and H446 cells,and the lowest uptake in H520 cells.HPDs are located in the cytoplasm of all the cell lines,showing a dotted distribution pattern.关键词
肺肿瘤/血卟啉衍生物/光化学疗法/细胞摄取/细胞内定位Key words
lung neoplasms/hematoporphyrin derivative/photochemotherapy/cellular uptake/intracellular localization分类
医药卫生引用本文复制引用
马亿江,丁晓倩,隋爱华,杨晓晖,曹艺巍,林存智..血卟啉衍生物在3种肺癌细胞系中的细胞摄取和定位[J].青岛大学学报（医学版）,2023,59(5):703-708,6.基金项目
北京科创医学发展基金会项目(KC2021-JX-0186-64) （KC2021-JX-0186-64）
