青岛大学学报（医学版）2023，Vol.59Issue(5)：709-713,5.DOI:10.11712/jms.2096-5532.2023.59.167
hUC-MSCs源性神经干细胞样细胞对大鼠脑缺血再灌注损伤的保护作用
THEPROTECTIVE EFFECT OF NEURAL STEM CELL-LIKE CELLS DERIVED FROM HUMAN UMBILICAL CORD MESENCHY-MAL STEM CELLS AGAINST CEREBRAL ISCHEMIA/REPERFUSION INJURY IN RATS
摘要
Abstract
Objective To investigate the protective effect of neural stem cell-like cells(NSCLCs)derived from human umbilical cord mesenchymal stem cells(hUC-MSCs)againstmiddle cerebral artery occlusion/reperfusion(MCAO/R)injury in rats.Methods The hUC-MSCs were induced into NSCLCs.A total of 30 male Sprague-Dawley rats weighing 250-280 g were randomly divided into sham-operation group(Sham group),model group(MCAO/R group),and treatment group(NSCLCs group).The suture method was used to establish a rat model of MCAO/R,and after modeling,the rats in the treatment group weretreated with NSCLCs transplantation via the external-internal carotid artery.The modified Neurological Severity Score(mNSS)was used to evaluate theneurobehavioral function of rats in each group,HE staining was used to observe neuronal mor-phology and structure,and immunohistochemical staining was used to measure the expression of neuronal Neurexin(NeuN).Results The MCAO/R group had a significant reduction in mNSS score compared with the Sham group,while the NSCLCs group had a significant increase compared with the MCAO/R group(F=5.119,P＜0.05).Compared with the Sham group,the MCAO/R group had a significant increase in the number of injured neuronal cells,and compared with the MCAO/R group,the NSCLCs group had a significant reduction in the number of such cells(F=173.928,P＜0.01).Compared with the Sham group,the MCAO/R group had a significant reduction in the number of NeuN-positive cells,and compared with the MCAO/R group,the NSCLCs group had a significant increase in the number of such cells(F=73.120,P＜0.01).Conclusion NSCLCs transplantation exerts a protective effect against cerebral ischemia/reperfusion injury in rats by improving neuron injury.关键词
神经干细胞/间质干细胞/脑缺血/再灌注损伤/神经保护/大鼠Key words
neural stem cells/mesenchymal stem cells/brain ischemia/reperfusion injury/neuroprotection/rats分类
医药卫生引用本文复制引用
王素平,钟亮,兰小磊,翟秋月,张睿..hUC-MSCs源性神经干细胞样细胞对大鼠脑缺血再灌注损伤的保护作用[J].青岛大学学报（医学版）,2023,59(5):709-713,5.基金项目
青岛市博士后基金项目(2018-80) （2018-80）
