青岛大学学报（医学版）2023，Vol.59Issue(5)：749-754,6.DOI:10.11712/jms.2096-5532.2023.59.146
Sirt6与狼疮性肾炎足细胞损伤的关系
RELATIONSHIP BETWEEN SIRT6 AND PODOCYTE INJURY IN PATIENTS WITH LUPUS NEPHRITIS
摘要
Abstract
Objective To investigate the relationship of silent information regulator 6(Sirt6,a deacetylase in the kidney tissue)with podocyte injury and disease activity in patients with lupus nephritis(LN)as well as the potential mechanism of podo-cyte injury in those patients.Methods Seventy treatment-naive patients with LN confirmed by percutaneous renal biopsy were selected and divided into active group(47 cases,≥7 points)and inactive group(23 cases,＜7 points)according to Systemic Lupus Erythematosus Disease Activity Index(SLEDAI),with another 15 patients who underwent nephrectomy due to renal trauma being enrolled into control group.The clinical data of all the patients were collected;the injury of kidney tissue and podocytes in the pa-tients was evaluated with a light microscope and an electron microscope;the expression levels of nephrin(a podocyte protein whose injury may lead to renal diseases)and Sirt6 in the kidney tissue were measured by immunohistochemistry;Spearman correlation analysis was used to determine the correlation between the expression of nephrin and laboratory test indices.Results Light mi-croscopy showed that the patients with LN had an increased glomerular volume,widened mesangial area,and increased mesangial cells and mesangial matrix,with greater changes in the active group.Electron microscopy showed that the control group had com-plete and regularly arranged glomerular foot processes,with continuous basement membrane;the LN group had obviously prolife-rated glomerular mesangial cells and mesangial matrix,unevenly thickened basement membrane,and diffusely fused,or even ab-sent,foot processes,with greater changes in the active group.Immunohistochemical staining showed that the expression of nephrin in the podocytes and Sirt6 in the kidney tissue was reduced in the LN group(with greater reductions in the active group than in the inactive group)compared with the control group.Spearman correlation analysis showed that the expression level of Sirt6 in the kid-ney tissue was negatively correlated with 24 h urinary protein quan-tification result(r=-0.580,P＜0.01),erythrocyte sedimentation rate(r=-0.559,P＜0.01),and SLEDAI score(r=-0.666,P＜0.01)and was positively correlated with complement C3(r=0.670,P＜0.01),serum albumin(r=0.660,P＜0.01),and nephrin ex-pression in the podocytes(r=0.954,P＜0.01).Conclusion The decrease in Sirt6 expression in the kidney tissue of patients with LN may be correlated with the podocyte injury and di-sease activity in these patients.关键词
狼疮肾炎/Sirtuin6/足细胞/免疫组织化学Key words
lupus nephritis/Sirtuin6/podocytes/immunohistochemistry分类
医药卫生引用本文复制引用
魏玉娇,陈超,周燕,刘南池,马瑞霞..Sirt6与狼疮性肾炎足细胞损伤的关系[J].青岛大学学报（医学版）,2023,59(5):749-754,6.基金项目
青岛市公共领域科技支撑计划项目(2012-1-3-2-(2)-nsh) （2012-1-3-2-(2）
青岛大学医学部"临床+X"工程科研项目(RZ-1900003244) （RZ-1900003244）
