山东农业科学2023，Vol.55Issue(11)：49-56,8.DOI:10.14083/j.issn.1001-4942.2023.11.008
离体培养条件下莱氏绿僵菌致病形态和非致病形态的转录组分析
Transcriptomic Analysis of Pathogenic and Nonpathogenic Forms of Metarhizium rileyi Cultured in vitro
摘要
Abstract
Metarhizium rileyi(Farlow)Kepler is an important entomopathogenic fungus that has both pathogenic and nonpathogenic forms during parasitism,but only the pathogenic form can kill the host.In order to understand the molecular mechanism of the transformation from nonpathogenic form to pathogenic form of M.rileyi,transcriptomic analysis of these two forms was performed.The results showed that a total of 89 498 Uni-genes were obtained from the two morphological fungus,and the proportion of genes related to"cellular com-ponent"in GO annotation was the highest,and also that related to"general function prediction only"in KOG annotation.However,for KEGG annotation,the genes involved in"metabolic pathways"in"metabolism"subclass and"MAPK signaling pathway"in"environmental information processing"subclass were the high-est,while in"cellular processes"subclass,the genes related to"quorum sensing"were also highly ex-pressed.These initially provided genetic evidence for quorum sensing regulation mechanism of the two morpho-logical transitions of M.rileyi.Differential expression gene(DEG)analysis showed that 3 113 genes were up-regulated and 3 671 genes were down-regulated during the morphological transformation of M.rileyi,but the difference enrichment analysis(DEGs)did not obtain effective results.In conclusion,the gene expression was significantly changed during morphological transformation of M.rileyi,and the metabolism was vigorous,quo-rum sensing mechanism was triggered and cell signal transduction pathway was activated,and finally the trans-formation from nonpathogenic form to pathogenic form was completed.This study could provide a useful refer-ence for in-depth understanding of the molecular mechanism of the M.rileyi pathogenicity.关键词
莱氏绿僵菌/转录组分析/致病形态/非致病形态/形态转变/离体培养Key words
Metarhizium rileyi/Transcriptomic analysis/Pathogenic form/Nonpathogenic form/Mor-phological transformation/In vitro culture分类
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刘守柱,张希鹏,郝轩卉,任宪銮,王世贤,聂玉恒,吴蕊..离体培养条件下莱氏绿僵菌致病形态和非致病形态的转录组分析[J].山东农业科学,2023,55(11):49-56,8.基金项目
山东省自然科学基金项目(ZR2020MC127) （ZR2020MC127）
聊城大学大学生创新创业训练计划项目(CXCY2023297) （CXCY2023297）
