山东农业科学2023，Vol.55Issue(11)：79-87,9.DOI:10.14083/j.issn.1001-4942.2023.11.012
不同需冷量枣品种休眠期间糖组分含量及相关酶活性变化规律
Change Rule of Sugar Component Content and Related Enzymes Activities of Jujube Cultivars with Different Chilling Requirements during Dormancy
摘要
Abstract
In order to explore the change rule of sugar accumulation and metabolism-related enzymes ac-tivities during dormancy of different jujube varieties with different chilling requirement,screen out key sub-stances affecting jujube tree dormancy and provide a theoretical basis for studying physiological mechanisms of jujube tree dormancy,this experiment was conducted using three jujube varieties of Tailihong with high chill-ing requirement,Fucuimi with medium chilling requirement,and Jingzao 39 with low chilling requirement as materials to analyze the changes of carbohydrate content and related metabolic enzymes activities of jujube shoots during dormancy and their relationship with dormancy.The results showed that the soluble sugar content in jujube branches during dormancy was the highest,ranging from 0.4%to 1.28%,followed by sucrose,glu-cose and fructose contents.Among the three jujube varieties,the sucrose content in Jingzao 39 branches reached the peak value of 0.63%on November 12,and that of Fucuimi and Tailihong reached the peak value on November 19,which was 0.45%and 0.36%,respectively.The sucrose content of Jingzao 39 dropped to the valley value of 0.11%on December 24,while that of Fucuimi dropped to the valley value of 0.05%on Decem-ber 31,and that of Tailihong dropped to the valley value of 0.08%on January 7.It could be seen that the peak and valley values of sucrose content corresponded to the time entering and releasing dormancy,which reflected that sucrose content changed with the process of dormancy.The neutral invertase(NI)activity of Jingzao 39,Fucuimi and Tailihong increased to the peak value on November 19,which were 20.02,15.60 and 16.73 μmol·g-1·min-1,respectively.The NI activity of Jingzao 39 dropped to the valley value of 12.60 μmol·g-1·min-1 on December 24,while that of Fucuimi decreased to the valley value of 12.34 μmol·g-1·min-1 on De-cember 31,and that of Tailihong decreased to the valley value of 11.36 μmol·g-1·min-1 on January 7.In line with the change trend of sucrose content,NI activity increased before dormancy,began to decline after ente-ring dormancy,and increased after dormancy was released;the generated valley value corresponded to the key time of dormancy release.In summary,the carbohydrates accumulation during the dormancy of jujube trees was mainly soluble sugar;sucrose was the key substance affecting jujube tree dormancy;the change of its con-tent would affect the process of jujube tree dormancy,and neutral invertase was the key enzyme for sugar accu-mulation during jujube tree dormancy.关键词
枣/需冷量/休眠/碳水化合物/代谢酶活性Key words
Jujube/Chilling requirement/Dormancy/Carbohydrates/Metabolic enzyme activity分类
农业科技引用本文复制引用
王超,张川疆,王振磊,吴翠云,林敏娟,于军..不同需冷量枣品种休眠期间糖组分含量及相关酶活性变化规律[J].山东农业科学,2023,55(11):79-87,9.基金项目
国家自然科学基金项目"枣树需冷量及休眠解除机制的研究"(31760553) （31760553）
兵团区域创新引导项目(2021BB009) （2021BB009）
塔里木大学校长基金项目"枣品种资源休眠生理的研究"(TDZKSS202261) （TDZKSS202261）
