山东医药2023，Vol.63Issue(29)：11-14,4.DOI:10.3969/j.issn.1002-266X.2023.29.003
青海地区汉族冠心病患者氯吡格雷代谢关键酶CYP2C19基因多态性分析
Genetic polymorphism analysis of CYP2C19 in Han Chinese patients with coronary heart disease in Qinghai area
摘要
Abstract
Objective By analyzing the gene polymorphisms of CYP2C19,a key enzyme for clopidogrel metabo-lism,in Han Chinese patients with coronary heart disease(CHD)in Qinghai region,in order to provide a reference for the individualized and precise treatment of clinical antiplatelet drugs.Methods We selected 403 cases of Han Chinese pa-tients with CHD in Qinghai region,collected patients'venous blood,and analyzed CYP2C19 gene sequences by real-time fluorescence quantitative PCR.According to the PCR results,we determined CYP2C19 genotypes and metabolic pheno-types.Hardy-Weinberg equilibrium test was used to analyze the data's group representativeness,and we compared the metabolic phenotypes of CYP2C19 among different regions in the relevant literature.Results The allele frequencies of CYP2C19*1,CYP2C19*2,CYP2C19*3,and CYP2C19*17 in 403 Han Chinese CHD patients were 60.55%,30.77%,5.70%,and 2.98%,respectively.And the frequencies of CYP2C19*1/*1,*1/*2,*1/*3,*2/*2,* 2/*3,*3/*3,*1/*17,*2/*17,*3/*17 genotypes were 40.2%,30.8%,6.7%,12.2%,4.0%,0.2%,3.2%,2.5%,and 0.2%,re-spectively.The Hardy-Weinberg equilibrium test showed that the CYP2C19 genotype frequencies of Han Chinese CHD pa-tients in Qinghai were in accordance with the Hardy-weinberg equilibrium(P<0.05),with a good representation of the group.The frequencies of different metabolic phenotype in the patients were as follows:13 cases of ultra fast metabolizer(3.20%),162 cases of extensive metabolizer(40.2%),162 cases of intermediate metabolizer(40.2%)and 66 cases of poor metabolizer(16.4%).There were significant differences in the distribution of metabolic phenotypes between Han Chinese CHD patients in Qinghai area and Han Chinese CHD patients in Beijing,Gansu,Ningxia,Shanxi and Guangzhou areas(all P<0.05).Conclusions The genotypes of Han Chinese CHD patients in Qinghai region were dominated by CYP2C19*1/*1 and CYP2C19*1/*2.Compared with other regions in China,the risk of clopidogrel resistance was higher in CHD patients in Qinghai region.关键词
基因多态性/冠状动脉粥样硬化性心脏病/氯吡格雷/CYP2C19/汉族/青海Key words
genetic polymorphism/coronary atherosclerotic cardiopathy/clopidogrel/CYP2C19/Han nationality/Qinghai region分类
医药卫生引用本文复制引用
李愈娴,刘世明..青海地区汉族冠心病患者氯吡格雷代谢关键酶CYP2C19基因多态性分析[J].山东医药,2023,63(29):11-14,4.基金项目
青海省卫生计生系统指导性计划课题项目(2020-wjzdx-87). （2020-wjzdx-87）
