山东医药2023，Vol.63Issue(29)：15-19,5.DOI:10.3969/j.issn.1002-266X.2023.29.004
子宫内膜癌组织LncRNA OGFRP1、TDRG1表达变化及其与PI3K/AKT信号通路和预后的关系
Expression changes of LncRNA OGFRP1 and TDRG1 in endometrial carcinoma and their relationships with PI3K/AKT signaling pathway and prognosis
摘要
Abstract
Objective To investigate the changes in the expression levels of long non-coding RNA(LncRNA)opi-oid growth factor receptor pseudogene 1(OGFRP1)and testicular development related gene 1(TDRG1)in endometrial carcinoma(EC)tissues,and their relationships with phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signal-ing pathway and prognosis.Methods Ninety patients with EC were selected.The expression levels of LncRNA OG-FRP1,TDRG1 and PI3K,AKT mRNA in EC tissues and paraneoplastic tissues of the patients were detected by RT-qP-CR.Pearson correlation was used to analyze the correlations between LncRNA OGFRP1,TDRG1 and PI3K,AKT mRNA expression in EC tissues.Based on the mean values of LncRNA OGFRP1 and LncRNA TDRG1 expression in EC tissues,patients were classified into those with high expression of LncRNA OGFRP1 and LncRNA TDRG1 and those with low ex-pression,and the survival curves of the patients with different expression of LncRNA OGFRP1 and LncRNA TDRG1 in EC tissues were plotted using the K-M method.The relationships between LncRNA OGFRP1 and LncRNA TDRG1 expression and patients'prognosis in EC tissues were analyzed by Log-Rank test.COX regression was used to analyze the factors af-fecting the prognosis of EC patients.Results LncRNA OGFRP1,LncRNA TDRG1,and PI3K and AKT mRNA expres-sion were higher in the EC tissues than in the paraneoplastic tissues(all P<0.05).Pearson correlation analysis showed that LncRNA OGFRP1 expression was positively correlated with PI3K mRNA and AKT mRNA expression levels in EC tis-sues(r=0.584,0.571,respectively;all P<0.01),and LncRNA TDRG1 expression was positively correlated with PI3K mRNA and AKT mRNA expression levels(r=0.593,0.584,respectively;all P<0.01).The 3-year overall survival rates of EC patients with high expression levels of LncRNA OGFRP1 and TDRG1 were lower than those of patients with low ex-pression levels of LncRNA OGFRP1 and TDRG1(all P<0.05).Cox regression analysis showed that federation internation-al of gynecology and obstetrics stage III,lymph node metastasis,LncRNA OGFRP1≥1.19,and LncRNA TDRG1≥1.35 were independent risk factors affecting the prognosis of patients with EC(all P<0.05).Conclusion LncRNA OGFRP1 and LncRNA TDRG1 expression levels increased in EC tissues,there were correlations between the expression levels of LncRNA OGFRP1 and LncRNA TDRG1 and expression levels of molecules related to the PI3K/AKT signaling pathway,and they were independent risk factors for poor prognosis of EC patients.关键词
长链非编码核糖核酸/阿片类生长因子受体假基因1/长睾丸发育相关基因1/PI3K/AKT信号通路/预后/子宫内膜癌Key words
long non-coding RNA/opioid growth factor receptor pseudogene 1/testis development related gene 1/PI3K/AKT signaling pathway/prognosis/endometrial carcinoma分类
医药卫生引用本文复制引用
邢堃,宋丹,康程..子宫内膜癌组织LncRNA OGFRP1、TDRG1表达变化及其与PI3K/AKT信号通路和预后的关系[J].山东医药,2023,63(29):15-19,5.基金项目
首都卫生发展科研专项项目(2020-2Z-06043). （2020-2Z-06043）
