山东医药2023，Vol.63Issue(32)：6-10,5.DOI:10.3969/j.issn.1002-266X.2023.32.002
HYOU1对高糖环境施万细胞自噬和凋亡的影响及其机制
Effects of HYOU1 on autophagy and apoptosis of Schwann cells in high glucose environment and its mechanism
摘要
Abstract
Objective To explore the effects of hypoxia up-regulated 1(HYOU1)on autophagy and apoptosis of Schwann cells in the high glucose environment and to explore the related mechanism based on ROS/PI3K/AKT pathway.Methods RSC96 cells were cultured,HYOU1 recombinant expression plasmid was constructed,and the cells were ran-domly divided into the high glucose group,high glucose + control plasmid group,and high glucose +HYOU1 plasmid group.Cells in the three groups were all cultured with high glucose,and then cells in the high glucose + control plasmid group and high glucose +HYOU1 plasmid group were transfected with control plasmid and pEGFP-HYOU1 plasmid,re-spectively.Western blotting was used to detect autophagy-related proteins(LC3,ATG5,ATG12,Beclin1,p62),JAK-STAT pathway-related proteins(JAK1,JAK2,STAT1,STAT3,STAT5,STAT6),and ROS/PI3K/AKT pathway-related proteins(PI3K,AKT,mTOR,pPI3K,pAKT,pmTOR).Flow cytometry was used to detect the apoptosis rate.The apop-tosis-related genes Bax and Bcl-2 were detected by RT-PCR,and the ROS level in each group was detected by DCFH-DA staining.Results Compared with the high glucose group and high glucose+ control group,LC3,ATG5,ATG12 and Be-clin1 protein levels decreased and p62 protein level increased in the high glucose+HYOU1 group(all P<0.05).Compared with the high glucose group and high glucose+ control group,the apoptosis rate and Bax mRNA expression decreased,while Bcl-2 mRNA expression and the protein expression levels of JAK1,JAK2,STAT1,STAT3,STAT5 and STAT6 in-creased in the high glucose+HYOU1 group(all P<0.05).Compared with the high glucose group and high glucose+ control group,PI3K,AKT,mTOR,pPI3K,pAKT and pmTOR protein levels increased,and ROS level decreased in the high glu-cose+HYOU1 group(all P<0.05).Conclusion HYOU1 overexpression can down-regulate autophagy and apoptosis of Schwann cells in the high glucose environment,and the mechanism may be related to the regulation of ROS/PI3K/AKT pathway.关键词
HYOU1基因/施万细胞/细胞自噬/细胞凋亡/ROS/PI3K/AKT通路/糖尿病/糖尿病周围神经病Key words
HYOU1 gene/Schwann cells/autophagy/apoptosis/ROS/PI3K/AKT pathway/diabetes mellitus/diabetic peripheral neuropathy分类
医药卫生引用本文复制引用
周晨明,薛岩,孟丽,朱艳,徐彦楠..HYOU1对高糖环境施万细胞自噬和凋亡的影响及其机制[J].山东医药,2023,63(32):6-10,5.基金项目
河北省医学科学研究课题计划项目(20220974). （20220974）
