山东医药2023，Vol.63Issue(32)：16-19,4.DOI:10.3969/j.issn.1002-266X.2023.32.004
丙酮酸钠溶液灌胃治疗糖尿病大鼠深Ⅱ°烧伤效果及其机制
Therapeutic effect of intragastric administration of sodium pyruvate solution on deep Ⅱ° scald in diabetic rats and analysis of its mechanism
摘要
Abstract
Objective To observe the therapeutic effect of intragastric administration of sodium pyruvate solution on deep Ⅱ° scald in diabetic rats and to analyze the possible mechanism.Methods Fifty male SD rats were randomly divid-ed into the control group,model group and experimental groups 1,2 and 3,with 10 rats in each group.The diabetic rat models were established by intraperitoneal injection of streptozotocin.After successful modeling,a deep partial thickness scald with diameter of 4 cm was inflicted on the back of each rat in the model group and the experimental groups.Rats in the experimental groups 1,2 and 3 were given 2.5%,7.5%and 10%sodium pyruvate solution by gavage,respectively,once a day,and rats in the control group and the model group were given the same amount of normal saline by gavage.On day 1,3,7,14,and 21 of drug administration,the wound healing rate was observed and calculated,and the body weight and blood glucose of rats were detected.Nicotinamide adenine dinucleotide(NAD+/NADH),malondialdehyde(MDA),superoxide dismutase(SOD),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the wound tissues were de-tected by ELISA.Results The wound healing rates of the model group on day 14 and 21 of administration were lower than those of the control group,and the wound healing rate of each experimental group on day 14 and 21 of administration was higher than that of control group,and the wound healing rate of the experimental group 3 was higher than those of the experimental groups 1 and 2(all P<0.05).The blood glucose of the model group was higher than that of the control group at each time point of administration,and the blood glucose of each experimental group was lower than that of the model group,and that of experimental group 3 was lower than those of experimental groups 1 and 2(all P<0.05).The body weight of the model group was lower than that of the control group from day 3 to 21 of administration,and the body weight of each experimental group was higher than that of the model group,and the body weight of the experimental group 3 was higher than that of the experimental groups 1 and 2 from day 7 to 21 of administration(all P<0.05).The levels of NAD+/NADH and SOD in the wound tissues after administration in the model group were lower than those in the control group,and those in each experimental group were higher than those in the model group,and those in experimental group 3 were higher than those in experimental groups 1 and 2(all P<0.05).The levels of MDA,IL-6,and TNF-α in wound tissue af-ter administration in the model group were higher than those in the control group,and those in each experimental group were lower than those in the model group,and those in experimental group 3 were lower than those in experimental groups 1 and 2(all P<0.05).Conclusion Intragastric administration of sodium pyruvate solution can promote wound healing,reduce blood glucose,and maintain normal body weight in diabetic rats with deep II° scald,and the mechanism may be re-lated to inhibiting inflammatory response and peroxidation damage of wound and improving energy metabolism.关键词
丙酮酸盐/深Ⅱ°烧伤/糖尿病/创面愈合Key words
pyruvate/deep Ⅱ° burns/diabetes mellitus/wound healing分类
医药卫生引用本文复制引用
弭倩,孟祥熙,田雨沫,何泽,孙奎..丙酮酸钠溶液灌胃治疗糖尿病大鼠深Ⅱ°烧伤效果及其机制[J].山东医药,2023,63(32):16-19,4.基金项目
河北省卫生健康委医学科学研究课题计划项目(20220009). （20220009）
