山东医药2023，Vol.63Issue(32)：30-33,4.DOI:10.3969/j.issn.1002-266X.2023.32.007
SLC5A8基因过表达的炎症性肠病细胞模型结构及IL-8、TGF-β1表达变化观察
Observation on changes in morphology of inflammatory bowel disease cell model and levels of IL-8 and TGF-β1 after SLC5A8 gene overexpression
摘要
Abstract
Objective To observe the changes in morphology of lipopolysaccharide-induced inflammatory bowel dis-ease cell model and levels of interleukin(IL)-8 and transforming growth factor(TGF)-β1 after SLC5A8 gene overexpres-sion.Methods Human normal colon epithelial cells were cultured and divided into the control group,model group and experimental group.Cells in the control group were transfected with pcDNA3.1-GFP empty plasmid,cells in the model group were transfected with pcDNA3.1-GFP empty plasmid and then were stimulated with 100 μg/mL lipopolysaccharide for 24 h,and the cells in the experimental group were transfected with overexpressed SLC5A8 gene plasmid and stimulated with 100 μg/mL lipopolysaccharide for 24 h.The structure of colonic epithelial cells in the three groups was observed un-der transmission electron microscope.IL-8 and TGF-β1 mRNA in cells of the three groups were detected by RT-qPCR,and IL-8 and TGF-β1 in the supernatant of culture medium were detected by ELISA.Results The microvilli on the cell mem-brane surface of the control group were not broken and exfoliated,and the tight junction structure was dense and banded;in the model group,the microvilli on the cell membrane surface became sparse,fell off,and the gap between the tight junc-tion cells widened;there was shedding of microvilli on the cell membrane surface of the experimental group,and the tight junction structure was partially destroyed.Compared with the control group,the expression of IL-8 mRNA and the level of IL-8 in the culture supernatant of the model group increased,while the expression of TGF-β1 mRNA and the level of TGF-β1 in the culture supernatant decreased(all P<0.01).Compared with the model group,the expression of IL-8 mRNA and the level of IL-8 in the culture supernatant of the experimental group were reduced,while the expression of TGF-β1 mRNA and the level of TGF-β1 in the culture supernatant increased(all P<0.01).Conclusion Overexpression of SLC5A8 gene helps maintain the morphology of lipopolysaccharide-induced inflammatory bowel disease cells,inhibit the expression of the inflammatory cytokine IL-8,and promote the expression of the anti-inflammatory cytokine TGF-β1,thereby reducing inflammatory reaction in inflammatory bowel disease.关键词
SLC5A8基因/白介素8/转化生长因子β1/炎症性肠病Key words
SLC5A8 gene/interleukin-8/transforming growth factor-β1/inflammatory bowel disease分类
医药卫生引用本文复制引用
赵泽满,吴育朔,刘雅涵,矫政洧,蔡思源,靳小石..SLC5A8基因过表达的炎症性肠病细胞模型结构及IL-8、TGF-β1表达变化观察[J].山东医药,2023,63(32):30-33,4.基金项目
河北省重点研发计划项目(2137773D). （2137773D）
