丝绸2023，Vol.60Issue(12)：28-35,8.DOI:10.3969/j.issn.1001-7003.2023.12.004
羊毛角蛋白融合型丝素蛋白膜材料的性能分析
Performance analysis of fibroin membrane materials mixed with wool keratin
摘要
Abstract
Silk has been used in China for thousands of years and is the only natural filament fiber in nature that can be mass-produced,which is of great significance in the textile industry.In recent years,protein films,a derivative material of silk,have attracted much attention and are widely used in the fields of antimicrobial dressings,bone repair scaffolds,slow-release carriers and biosensors,and have become a high-tech material with a promising future.Despite the superior mechanical properties of native silk,the strength and toughness of regenerated silk-protein membranes are unsatisfactory,which greatly hinders their wide application.To enhance the mechanical strength of regenerated sericin materials,some scholars have enhanced the mechanical strength of regenerated sericin materials by mixing natural proteins,such as wool keratin and spider silk protein,and also improved the softness and ductility of regenerated sericin materials by adding non-protein components,such as glycerol and polyvinyl alcohol,of which glycerol can also promote the interactions between the molecules of sericin and the water molecules to improve the hydrophilicity of sericin.Both protein and non-protein addition methods are often accompanied by problems such as complex dissolution,cumbersome process,waste of resources and environmental pollution. Wool keratin-associated proteins(KAPs)are fibrous proteins rich in cysteine,capable of forming disulphide bonds upon oxidation.These bonds,along with hydrophobic interactions and van der Waals forces,contribute to the high mechanical strength and structural stability of keratin.In this study,KAP-incorporated silk was generated through transgenic methods and used as the material.Silk protein membranes were then obtained by gradually removing water from the silk protein solution and shaping it accordingly.Furthermore,1％glycerol was added to the silk solution to modify the protein membrane.We tested the secondary structure,physicochemical properties,and mechanical properties of ordinary sericin protein membranes,KAP-incorporated sericin protein membranes,and glycerol-plasticized protein membranes,and then analyzed the improvement of sericin protein membranes by the incorporation of KAP. In this study,we used the KAP-incorporated silk obtained by transgenic transfer of the wool keratin gene into the silkworm,Bombyx mori,to produce performance-enhanced hybrid silk-protein membranes.Compared with the tedious process of dissolving silk and wool separately and then mixing them in the traditional method of making hybrid protein membranes,this method has the advantages of relative simplicity and higher efficiency,and provides a new way and idea for the research and utilisation of natural proteins such as silk and wool. The KAP-incorporated sericin protein membranes produced were tested,and it was found that the incorporation of KAP protein could increase the secondary structure of the stability of silk fibroin membranes and improve the mechanical properties of the membranes,and the adding of glycerol could also significantly improve the tensile properties and hydrophilicity of the KAP sericin membranes. The production of silk/wool keratin mixed protein films through transgenic methods has the characteristics of simplicity,zero pollution,and excellent performance,which can provide new ideas for the research and application of natural silk materials.关键词
丝素/羊毛角蛋白/再生蛋白膜/结构/机械性能/亲水性Key words
fibroin/wool keratin/regenerated protein membrane/structure/mechanical property/hydrophilic分类
轻工纺织引用本文复制引用
常雪宁,何海波,王依辰,曾頔,代方银,赵天福..羊毛角蛋白融合型丝素蛋白膜材料的性能分析[J].丝绸,2023,60(12):28-35,8.基金项目
国家现代农业产业技术体系项目(CARS-18-ZJ102) （CARS-18-ZJ102）
家蚕分子育种法创制种质资源的快速纯系化技术探究项目(20220412204853557) （20220412204853557）
