世界中医药2023，Vol.18Issue(19)：2739-2745,2751,8.DOI:10.3969/j.issn.1673-7202.2023.19.005
荆防颗粒对脂多糖致小鼠急性肺损伤的预防和保护作用
Preventive and Protective Effects of Jingfang Granules on Lipopolysaccharide-induced Acute Lung Injury in Mice
摘要
Abstract
Objective:To study the preventive and protective effects of Jingfang Granules on lipopolysaccharide(LPS)-induced a-cute lung injury in mice,and to preliminarily explore the mechanism.Methods:Male C57BL/6 mice were randomly divided into control group,model group,and Jingfang Granules low-,medium-,and high-dose groups(1.25 g/kg,2.5 g/kg,and 5 g/kg,respec-tively),10 in each group.Mice in each group were separately given normal saline of the same volume and Jingfang Granules of cor-responding concentrations.After 6 d of administration,the model of acute lung injury was established by endotracheal infusion of LPS in model group and Jingfang Granules groups,while the control group received the same volume of normal saline by endotra-cheal infusion.The macroscopic morphological changes of lung tissues in each group were analyzed.HE staining was used to ob-serve the pathological changes of lung tissues and the pathological score was given.Monocyte chemotactic protein 1(MCP-1),inter-leukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in lung tissues were detected.Oxidative stress indi-cators such as myeloperoxidase(MPO),inducible nitric oxide synthase(iNOS),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione(GSH)were also detected.Results:Compared with the the conditions in control group,MCP-1,TNF-α,IL-1β,IL-6,MPO,iNOS and MDA in the lung tissues of mice in model group as well as the pathological score were increased(P＜0.01),with severe macroscopic morphological changes,and the activities of SOD and GSH were decreased(P＜0.01).Compared with model group,Jingfang Granules groups had down-regulated MCP-1,TNF-α,IL-1 β,IL-6,MPO,iNOS and MDA to different de-grees(P＜0.05),and increased activities of SOD and GSH(P＜0.05).The pathological score of lung tissues was lowered(P＜0.01),and the macroscopic morphology was improved.Conclusion:Jingfang Granules can inhibit LPS-induced acute lung injury to a certain extent and improve the macroscopic morphology and pathological changes of lung tissues.The mechanism may be related to inhibiting the inflammatory factors,reducing the oxidative stress,and enhancing the antioxidant capacity of the body.关键词
荆防颗粒/脂多糖/急性肺损伤/炎症介质/抗炎/氧化应激/抗氧化Key words
Jingfang Granules/Lipopolysaccharide/Acute lung injury/Inflammatory factors/Anti-inflammation/Oxidative stress/Antioxidation分类
医药卫生引用本文复制引用
赵玉升,张贵民,张盈,程国良,姚景春,曹天佑,吴同,孔慧,屈会化,赵琰..荆防颗粒对脂多糖致小鼠急性肺损伤的预防和保护作用[J].世界中医药,2023,18(19):2739-2745,2751,8.基金项目
2021年岐黄学者支持项目(90020163320012) （90020163320012）
北京中医药大学横向课题研究项目(2180071720113) （2180071720113）
