色谱2023，Vol.41Issue(12)：1062-1072,11.DOI:10.3724/SP.J.1123.2023.04026
在线样品制备技术耦合液相色谱-质谱系统在食品危害物检测中的应用进展
Application progress of on-line sample preparation techniques coupled with liquid chromatography-mass spectrometry system in the detection of food hazards
摘要
Abstract
Food safety has received increased attention,and food detection is of great signifi-cance.The food matrix is complex,and diverse food hazards have been identified.Thus,the detection methods and sample preparation techniques for food matrices must be continuously optimized and updated.Several steps are usually required when a chromatographic system is used to determine food hazards:sample preparation,that is,the separation of targets from dif-ferent substrates using a suitable preprocessing method and target-substance separation and purification,which is usually achieved using chromatographic separation.The selection of an appropriate detector for qualitative and quantitative analyses is usually based on the properties of the target compound.The sample preparation procedure is considered the most time-consu-ming aspect of the entire food-analysis process.It is also prone to analytical errors.Therefore,optimization of the sample preparation process is a key issue in the field of chemical analysis.Researchers have developed a series of new,efficient,and accurate sample preprocessing methods,and an on-line sample-preparation system has been found to be a feasible approach.On-line sample preparation coupled with liquid chromatography-mass spectrometry(LC-MS)presents many advantages.First,manual operation could reduce analytical errors to ensure good accuracy and repeatability.It could also reduce the consumption of chemical reagents and avoid cross-contamination between samples.Furthermore,an on-line sample-preparation sys-tem could shorten the sample-preparation time and improve the detection efficiency.On-line sample preparation coupled with LC-MS has been widely applied in the fields of environment,biology,and food.On-line sample preparation systems coupled with LC-MS are divided into two modules:the first modules involves sample preparation and the second module involves the LC system.The first module remove impurities and isolates the target compounds in preparation for their qualitative and quantitative detection.The coupling of these two modules depends mainly on valve switching. In this paper,we introduce the most frequently used on-line sample-preparation techniques,including on-line solid phase extraction(on-line SPE),in-tube solid phase microextraction(in-tube SPME),and turbulent chromatography(TFC).We then describe the basic principles and coupling equipment of these three on-line analytical technologies in detail.The coupling equip-ment establishes a physical connection between the two modules.Next,we discuss the proper-ties of different purification fillers in an on-line sample-preparation column.The applications and research progress of on-line systems for pesticide residues,veterinary drug residues,and biotoxins are also discussed.Compared with offline sample preparation,on-line analytical sys-tems present several advantages.On-line analytical systems can not only greatly reduce the analysis time and solvent consumption but also improve the detection sensitivity and accuracy.Such systems can be used to determine food hazards to ensure food safety.Finally,the existing problems and development trends of on-line analytical systems are discussed and prospected.To promote the applications of on-line analytical technology in food-safety detection,we sug-gest that the following three aspects be considered.First,more on-line purification columns with novel fillers,in addition to C18 or polymer fillers,should be developed.Second,com-pared with ordinary detectors,high-resolution MS detectors have better precision and accuracy.Coupling on-line analytical technologies with a high-resolution mass spectrometer may be bene-ficial for the further development of on-line analyses.Third,different food matrices should be compared and evaluated to continuously optimize the detection process and improve the effi-ciency of on-line analytical systems.As concerns regarding food safety issues have increased,the applications of on-line analytical technologies for food detection can be expected to become increasingly important.关键词
在线样品制备技术/在线固相萃取/管内固相微萃取/湍流色谱法/食品危害物/综述Key words
on-line sample preparation techniques/on-line solid phase extraction(on-line SPE)/in-tube solid phase microextraction(in-tube SPME)/turbulent flow chromatography(TFC)/food hazards/review分类
化学化工引用本文复制引用
翟洪稳,马红玉,曹梅荣,张明星,马俊美,张岩,李强..在线样品制备技术耦合液相色谱-质谱系统在食品危害物检测中的应用进展[J].色谱,2023,41(12):1062-1072,11.基金项目
河北省市场监督管理局科研计划项目(2021ZD17) （2021ZD17）
国家市场监督管理总局科技计划项目(2020MK014) （2020MK014）
河北省高层次人才资助项目(A202101055,C20221051). Research Project of Hebei Administration for Market Regulation(No.2021ZD17) （A202101055,C20221051）
Science and Technology Project of State Administration for Market Regulation(No.2020MK014) （No.2020MK014）
Hebei Province High Level Talent Funding Program(Nos.A202101055,C20221051). （Nos.A202101055,C20221051）
