生物加工过程2023，Vol.21Issue(6)：599-607,9.DOI:10.3969/j.issn.1672-3678.2023.06.002
酵母中乙酰辅酶A的代谢工程研究进展
Advance in metabolic engineering of acetyl-CoA in yeast
摘要
Abstract
Acetyl-CoA,a key node in microbial metabolic pathways,is involved in the intracellular biosynthesis of a variety of chemicals.The modification of production pathway of acetyl-CoA through metabolic engineering methods can potentially increase the acetyl-CoA level,thus becomes an important approach to improve the yields of compounds with acetyl-CoA as a synthetic precursor.In this paper,we first presented various pathways of the intracellular acetyl-CoA synthesis,including PDH bypass pathway,PDH pathway,PK-PTA pathway and citrate cleavage pathway,as well as their differences in Saccharomyces cerevisiae and Yarrowia lipolytica.Then we summarized several strategies for the metabolic regulation of acetyl CoA synthesis pathway in yeast,along with their comparison in two kinds of yeast.This review will provide useful guidance for the efficient production of target compounds in yeast.关键词
乙酰辅酶A/酵母/代谢工程Key words
acetyl-CoA/yeast/mtabolic engineering分类
生物学引用本文复制引用
王招悬,刘营航,刘萌萌,祁庆生..酵母中乙酰辅酶A的代谢工程研究进展[J].生物加工过程,2023,21(6):599-607,9.基金项目
国家重点研发计划(2021YFC2100500) （2021YFC2100500）
山东省重大创新工程项目(2020CXGC010602) （2020CXGC010602）
