生物加工过程2023，Vol.21Issue(6)：616-622,7.DOI:10.3969/j.issn.1672-3678.2023.06.004
优化稳定剂提高丙酮酸氧化酶稳定性的研究
Enhancement of pyruvate oxidase stability by chemical stabilizers
摘要
Abstract
Aerococcu sviridans pyruvate oxidase was widely utilized in various analysis and diagnosis in food and medical industry,but it suffered from low stability because of its structure formed with multimeric subunits.To prolong the shelf life of pyruvate oxidase,chemical stabilizers were usually added into pyruvate oxidase solution to improve its stability.Multiple potential stabilizers,including alcohols,sugars,ions,and various combinations,were selected in our screening experiments.The optimized conditions,with a mixture of 0.01 mmol/L copper sulfate,30 g/L glycerol and 90 g/L PEG2000,could significantly improve the stability of pyruvate oxidase,and the final pyruvate oxidase activity was increased to 1.5 times at 4 ℃,pH 6.0.Such improvement of pyruvate oxidase stability was attributed to the enhanced interactions of subunits in the tetramer structure of pyruvate oxidase.Notably,pyruvate oxidase with stabilizers could maintain 46.7％of enzymatic activity after it was stored at-20 ℃ for 28 days.Our research provided a general guidance how to improve the stability of pyruvate oxidase,which could benefit the application of pyruvate oxidase.关键词
丙酮酸氧化酶/酶稳定性/稳定剂/多聚酶/优化Key words
pyruvate oxidase/enzyme stability/stabilizers/multimeric enzyme/optimization分类
化学化工引用本文复制引用
刘冰,丛文杰,姚兵莉,周化岚,张建国..优化稳定剂提高丙酮酸氧化酶稳定性的研究[J].生物加工过程,2023,21(6):616-622,7.基金项目
国家自然科学基金(31870045) （31870045）
