生物加工过程2023，Vol.21Issue(6)：623-631,9.DOI:10.3969/j.issn.1672-3678.2023.06.005
用壳寡糖修饰的方法提高酵母转化酶的催化活性
Enhancement of yeast invertase activity through chemical modification with chitooligosaccharide
摘要
Abstract
The yeast invertase is often modified for the industrial catalysis and medicine synthesis.Herein,chitooligosaccharide(COS)was used to react with the amino groups of yeast invertase(YInv),affording a modified invertase(named COS-Inv).As a result,COS-Inv showed much larger molecular weight than the natural yeast invertase(YInv),and the saccharide contents in YInv and COS-Inv were 2.75 and 6.34 mg/mg,respectively.Compared with YInv,the catalytic activity of COS-Inv was greatly improved by 200.3％,and COS-Inv displayed much lower Km than YInv(58.92 mmol/L vs 125.45 mmol/L).According to the three-dimensional fluorescence spectra,the fluorescence intensity of COS-Inv was much lower than that of YInv,indicating the different three-dimensional molecular structures for COS-Inv and YInv.Our results demonstrated that the modification of yeast invertase by chitooligosaccharide could serve as an effective way to improve its catalytic activity.关键词
酵母转化酶/壳寡糖/化学修饰/酶反应动力学Key words
yeast invertase/chitooligosaccharide/chemical modification/enzyme reaction kinetics分类
生物科学引用本文复制引用
黎春怡,黄卓烈,巫光宏,何平,詹福建..用壳寡糖修饰的方法提高酵母转化酶的催化活性[J].生物加工过程,2023,21(6):623-631,9.基金项目
国家自然科学基金(10074016) （10074016）
