生物加工过程2023，Vol.21Issue(6)：642-651,10.DOI:10.3969/j.issn.1672-3678.2023.06.007
竹黄菌漆酶对工业染料的脱色降解及其基因分析
Shiraia laccase:decolorization of industrial dyes and its gene analysis
摘要
Abstract
The Shiraia stromas or fruiting bodies are used as traditional Chinese medicine(TCM)to invigorate blood circulation and relieve rheumatic pains.Due to the existence of hypocrellins as the major bioactive components,they can also serve as potential photosensitizers with anti-cancer and anti-viral activities.Shiraia sp.S8,which was isolated from the short tissues of Brachystachyum densiflorum and its mycelium culture,could produce extracellular laccase in the medium.The crude laccase in cultural broth could reach its peak activity of 1 361 U/L on the 8th day.The crude laccase showed good stability at pH 6.0-7.0 as＞50％laccase activity was maintained after 108 h under this pH.The optimal pH and temperature for the enzymatic activity of laccase turned out to be pH 4.0 and 60 ℃,respectively.The decolorization of industrial dyes,including reactive blue 19,malachite green and neutral red,was carried out with the crude laccase under the mediation by 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate)(ABTS)at 33.33 µmol/L or acetosyringone(AS)at 266.66 μmol/L.As a result,the decolorization percentages in 60 min reached 85.06％,88.55％and 81.13％,respectively.Based on the transcriptome data of the fungus,the full-length cDNA(1 785 bp)was cloned for laccase and named as lcc1 with 1 002 bp in open reading frame(ORF).The protein encoded by lcc1 would be composed of 594 amino acid residues,including a complete conserved domain(first 18 amino acid residues in the N-terminal)of fungal laccase.In addition,the isoelectric point of this enzyme would be around 6.05 and the calculated molecular weight was about 6.53 × 104.This study demonstrated the decolorization of industrial dyes by crude laccase and provided gene analysis for such a novel strain of fungal enzyme.关键词
竹黄菌/漆酶/染料脱色/基因分析/Brachystachyum densiflorumKey words
Shiraia/laccase/dye decolorization/gene analysis/Brachystachyum densiflorum分类
生物学引用本文复制引用
郭艳华,王玥,李信萍,周建芹,王剑文,郑丽屏..竹黄菌漆酶对工业染料的脱色降解及其基因分析[J].生物加工过程,2023,21(6):642-651,10.基金项目
国家自然科学基金(82073955、81773696) （82073955、81773696）
江苏高校优势学科建设工程(RAPD) （RAPD）
