生物技术通报2023，Vol.39Issue(11)：205-216,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0722
大肠杆菌对木质纤维素水解液抑制物的胁迫耐受性
Stress Tolerance of Escherichia coli to Inhibitors in Lignocellulosic Hydrolysates
摘要
Abstract
Lignocellulosic biomass(LCB)is a promising alternative to fossil material,the biofuels,biochemicals and biomaterials are produced via its biorefinery,which may reduce carbon emissions,contributing to achieve the carbon peaking and carbon neutrality goals.Therefore,LCB is receiving more and more attentions.However,there are multiple steps involved in lignocellulosic biorefinery including pretreatment,microbial fermentation and product purification,in which,various compounds generated from pretreatment of LCB inhibit cell growth and fermentation performance of microbes,which is one of the bottlenecks of bioconversion efficiency.Escherichia coli is a commonly used host for lignocellulosic biorefinery and extensively used for the production of many compounds,thus,it is of great importance to study the stress tolerance of E.coli to inhibitors in lignocellulosic hydrolysates for improving lignocellulosic biorefinery efficiency.This paper first introduced the main components and structures of lignocellulose,and briefly elucidated pretreatment methods to lignocellulose as well as main inhibitors in the hydrolysate after pretreatment.Then the paper summarized the toxic effects of main inhibitor furans,carboxylic acids and phenolics in the hydrolysate of lignocellulose on E.coli,as well as the mechanisms of stress tolerance against these inhibitors and the engineering targets for improving strain tolerance based on the mechanisms.Finally,the paper reviewed the strategies for strain engineering to improve the tolerance of E.coli to above motioned inhibitors,including random mutagenesis,adaptive laboratory evolution and omics-assisted rational design,,aiming to provide references for metabolic engineering of efficient E.coli strains for lignocellulosic biorefinery.关键词
木质纤维素水解液抑制物/胁迫耐受性/大肠杆菌/菌株改造/呋喃类/羧酸类/酚类Key words
inhibitors in lignocellulosic hydrolysate/stress tolerance/Escherichia coli/strain engineering/furans/carboxylic acids/phenolics引用本文复制引用
唐瑞琪,赵心清,朱笃,汪涯..大肠杆菌对木质纤维素水解液抑制物的胁迫耐受性[J].生物技术通报,2023,39(11):205-216,12.基金项目
国家重点研发项目(2021YFC2101303-4),国家重点研发计划(2022YFE0108500),江西省自然科学基金项目(20232BAB205008),江西科技师范大学博士科研启动基金(2022BSQD11) （2021YFC2101303-4）
