生物技术通报2023，Vol.39Issue(11)：217-225,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0123
一种耐盐复合菌剂的制备和促生作用研究
Preparation of Compound Halotolerant Bioinoculant and Study on Its Growth-promoting Effect
摘要
Abstract
Soil salinization is a major factor seriously limiting crop growth and yield.Using soil beneficial microorganisms for saline field amendment is the reliable and sustainable approach.Two halotolerant bacteria strains,Microbacterium oxydans C8 and Stenotrophomonas maltophilia B4,were isolated from saline field in our previous study.Strain C8 has the ability to dissolve potassium,organic and inorganic phosphorus as well as produce auxin.Strain B4 is capable of solubilizing organic phosphorus and producing auxin.In this paper,the effect of mixed culture of C8 and B4 on their functions was studied.The results showed that the mixed culture of C8 and B4 demonstrated significantly stronger ability of dissolving potassium,phosphorus and produce auxin than a single strain.Orthogonal experiments and response surface test were used to optimize the fermentation medium and condition of C8 and B4 mixture,the results showed that the optimized fermentation medium was as follows:glucose 10 g/L,yeast extract 10 g/L,sodium chloride 4.5 g/L.The optimized fermentation conditions were:pH 7.4,temperature 28.8℃,rotating speed 129 r/min,inoculation amount 2%,the liquid content 20%and culture time 23 h.Using tobacco as material,the growth-promoting effects of compound bioinoculant of C8 and B4 were studied.The results showed that,the compound bioinoculant significantly prompted plant growth under salt stress.关键词
耐盐促生混合菌剂/发酵优化/烟草/促生作用Key words
salt-tolerant and growth-promoting bacteria inoculant/optimization of fermentation/tobacco/growth-promoting effect引用本文复制引用
车永梅,刘广超,郭艳苹,叶青,赵方贵,刘新..一种耐盐复合菌剂的制备和促生作用研究[J].生物技术通报,2023,39(11):217-225,9.基金项目
国家自然基金委联合项目(U2106229),山东省现代农业产业技术体系(SDAIT-25-05),山东省良种工程项目(2020LZGC008) （U2106229）
