生物技术通报2023，Vol.39Issue(11)：226-237,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0417
耐高温酿酒酵母的构建与高温耐受机制解析
Construction and Mechanism Analysis of High-temperature Resistant Saccharomyces cerevisiae
孙言秋 1谢采芸 2汤岳琴2
作者信息
- 1. 四川大学建筑与环境学院环境生物技术研究中心,成都 610065
- 2. 四川大学建筑与环境学院环境生物技术研究中心,成都 610065||四川省环境保护有机废弃物资源化利用重点实验室,成都 610065
- 折叠
摘要
Abstract
This work aims to construct Saccharomyces cerevisiae strains with excellent high-temperature tolerance and investigate the high-temperature tolerance mechanisms of the strains.CRISPR/Cas9 technology was used to knock out ASP3 in flocculating industrial S.cerevisiae strain KF-7 and further highly expressed CRZ1(Encoding transcription factor Crz1p with zinc finger structure).And the high-temperature tolerance mechanism of the recombinant strains was revealed through comparative transcriptomic analysis.The results showed that the ASP3-knockout strain KAS11 utilized 98.36 g/L glucose and produced 43.68 g/L ethanol at 44℃.After CRZ1 high expression based on KAS11,strain KASCR7 produced 48.02 g/L ethanol from 105.37 g/L glucose.Compared with KF-7,the ethanol production of the two recombinant strains increased by 4.77%and 15.18%,respectively.Comparative transcriptomic analysis revealed that genes involved in ribosome biogenesis and translation significantly repressed in the recombinant strains under high-temperature stress.In contrast,heat shock protein genes as well as genes involved in biosynthesis of NAD+,NADH,purine,glycerol,and proline were significantly induced.These responses may collectively lead to the enhanced high-temperature tolerance of the recombinant strains.The results may provide excellent strain resources and theoretical basis for the construction of excellent high-temperature tolerant S.cerevisiae strains.关键词
酿酒酵母/燃料乙醇/胁迫耐受/高温发酵/比较转录组Key words
Saccharomyces cerevisiae/fuel ethanol/stress tolerance/high-temperature fermentation/comparative transcriptome引用本文复制引用
孙言秋,谢采芸,汤岳琴..耐高温酿酒酵母的构建与高温耐受机制解析[J].生物技术通报,2023,39(11):226-237,12.
