生物技术通报2023，Vol.39Issue(11)：283-296,14.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0531
单叶蔷薇NAC基因家族鉴定及干旱胁迫响应分析
Identification of the NAC Gene Family in Rosa persica and Response Analysis Under Drought Stress
摘要
Abstract
NAC transcription factors play a crucial role in plant stress response,and the study of the drought tolerance of Rosa persica,which is mainly distributed in Xinjiang Uygur Autonomous Region,China,is of great importance for the management of ecological problems in desert areas.Genome-wide identification of the NAC gene family in R.persica was performed,the gene family characteristics and expression patterns were analyzed,and the NAC gene expression under drought stress was validated using real time quantitative PCR(RT-qPCR).The NAC gene family had a highly variable gene profile,while the gene structure and motifs were relatively conserved.Promoter analysis indicated that RbeNAC had an important role in the regulation of hormone synthesis and adaptation to environmental stresses.Analysis of transcriptome-based data revealed that RbeNAC gene expression demonstrated tissue specificity and response to drought stress.RT-qPCR results indicated that seven genes(RbeATAF,RbeSOG1,RbeNAC17,RbeNAC71,RbeNAC72,RbeNAC90 and RbeNAC96)in the roots and leaves,responded positively to drought stress.In this study,the NAC genes of R.persica were identified,the feasible functions of different genes were explored,and seven genes that respond positively to drought stress were found,which may provide a reference for breeding for stress resistance in R.persica.关键词
单叶蔷薇/NAC转录因子/干旱胁迫/基因表达Key words
Rosa persica/NAC transcription factors/drought stress/gene expression引用本文复制引用
冯策婷,江律,刘鑫颖,罗乐,潘会堂,张启翔,于超..单叶蔷薇NAC基因家族鉴定及干旱胁迫响应分析[J].生物技术通报,2023,39(11):283-296,14.基金项目
城乡人居生态环境学(北京高校高精尖学科建设项目) （北京高校高精尖学科建设项目）
