生物技术通报2023，Vol.39Issue(11)：328-339,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0049
异子蓬SabHLH169基因的克隆及抗旱功能分析
Cloning of Basic Helix-loop-helix(bHLH)Transcription Factor Gene SabHLH169 in Suaeda aralocaspica and Analysis of Its Resistances to Drought Stress
摘要
Abstract
The basic helix-loop-helix(bHLH)transcription factor(TF)is one of the largest TF families in eukaryotes,its members are widely involved in plant growth,development and response to stress.In our previous work of studying photosynthesis key enzyme PEPC-1 in Suaeda aralocaspica,a bHLH TF(SabHLH169)potentially interacting with the SaPEPC-1 promoter was screened.In the present study,the biological characteristics and preliminary function of this gene was explored,which may provide evidence for further studies of bHLH related genes in S.aralocaspica.The gene was obtained by homogous cloning,and the drought-resistant properties of SabHLH169 gene were characterized by bioinformatics and qRT-PCR analyses.The results showed that the open reading frame(ORF)of SabHLH169 contained 2100 bp nucleotides,encoding an protein containing 699 amino acids.The protein had a typical bHLH structural domain at the C-terminus.The prokaryotic expression and Western blot of the recombinant protein showed that the protein was expressed correctly,and the size was consistent with the predicted result.Arabidopsis thaliana transgenic lines of SabHLH169 presented higher drought tolerance at germination stage.Under drought stress,three drought-responsive genes(AtRD22,AtRD29A and AtDREB2A)and three photosynthesis key enzyme genes(AtLhcb2,AtRubicso and AtPEPC)were up-regulated in transgenic A.thaliana.Meanwhile,the PEPC enzyme activity was significantly enhanced.It is speculated that SabHLH169 gene may be involved in response to drought stress.关键词
异子蓬/bHLH/干旱胁迫/转基因拟南芥/光合关键酶Key words
Suaeda aralocaspica/bHLH/drought stress/transgenic A.thaliana/photosynthesis key enzyme引用本文复制引用
鄢梦雨,韦晓薇,曹婧,兰海燕..异子蓬SabHLH169基因的克隆及抗旱功能分析[J].生物技术通报,2023,39(11):328-339,12.基金项目
国家自然科学基金项目(32160057) （32160057）
