生物技术通报2023，Vol.39Issue(11)：340-349,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0624
金针菇Ⅱ类过氧化物酶基因在子实体发育与胁迫应答过程的表达特征
Characteristics of Class Ⅱ Peroxidase Gene Expression During Fruiting Body Development and Stress Response in Flammulina filiformis
摘要
Abstract
Class Ⅱ peroxidase(CIIp)is an important antioxidant enzyme in active oxygen metabolism,and participates in the oxidative response of organism.Based on the genomic data of Fammulina filiformis,two Class Ⅱ peroxidase genes were identified and named as FfCIIp1 and FfCIIp2,respectively.Online software was used for bioinformatics analysis of the genes,and real-time fluorescence quantitative PCR was used to analyze the expression patterns of genes in different fruit body tissues and stress responses of F.filiformis.The results showed that the full length of FfCIIp1 was 1 638 bp,including a complete open reading frame of 1 131 bp,encoding 376 amino acids.FfCIIp2 was 1 410 bp in length,containing a complete open reading frame of 1 032 bp and encoding 343 amino acids.Conserved domain and phylogenetic analysis showed that FfCIIp1 and FfCIIp2 belonged to the manganese peroxidase and generic peroxidase families,respectively.RT-qPCR results showed that both FfCIIp genes were highly expressed in the stipe during the elongation stage,and significantly up-regulated in the stipe of fast elongation region.FfCIIp1 and FfCIIp2 were up-regulated by both injury and oxidative stress,and the up-regulated range of FfCIIp2 was significantly higher than FfCIIp1.These results indicate that two CIIp family genes are involved in stipe elongation and stress response,and FfCIIp2 is more sensitive to injury and oxidative stress of F.filiformis.关键词
金针菇/锰过氧化物酶/通用过氧化物酶/子实体发育/胁迫应答/表达分析Key words
Flammulina filiformis/manganese peroxidase/generic peroxidase/fruiting body development/stress response/expression analysis引用本文复制引用
刘媛媛,魏传正,谢永波,仝宗军,韩星,甘炳成,谢宝贵,严俊杰..金针菇Ⅱ类过氧化物酶基因在子实体发育与胁迫应答过程的表达特征[J].生物技术通报,2023,39(11):340-349,10.基金项目
中国农业科学院科技创新工程项目(34-IUA-06),四川省联合育种攻关项目,国家自然科学基金青年科学基金项目(32102458) （34-IUA-06）
