土壤2023，Vol.60Issue(5)：1025-1034,10.DOI:10.13758/j.cnki.tr.2023.05.012
有机肥施用对植烟土壤氮素矿化及土壤酶和微生物群落的影响
Effects of Organic Fertilizers on Nitrogen Mineralization,Soil Enzyme Activities and Microbial Communities in Tobacco-planting Soil
摘要
Abstract
In order to explore the effects of different organic fertilizers on nitrogen mineralization,soil enzyme activities and soil microbial communities in tobacco-planting soil,a lab culture experiment was conducted with different organic fertilizers(plant-source organic fertilizer,sesame cake fertilizer,biochar organic fertilizer)under the condition of equal nitrogen(100 mg/kg)input and 30℃,the changes of nitrate nitrogen and ammonium nitrogen contents,soil enzyme activities and bacterial community diversities were analyzed in different culture periods.The results show that different organic fertilizers increase soil mineral nitrogen content,nitrogen mineralization amount and rate are the highest under cake fertilizer in the early culture stage,while the highest under biochar organic fertilizer in the later culture stage.Biochar and plant-source organic fertilizers significantly improve the activities of soil urease and nitrate reductase,while sesame cake fertilizer significantly improves the activities of protease and sucrase.Compared with no organic fertilizer treatment(CK),organic fertilizers significantly increase soil bacterial diversity,the dominant bacteria phyla are Proteobacteria,Acidobacteria,Bacteroidetes and Planctomycetes,and the dominant bacteria genera are Sphingomonas and RB41.Sphingomonas is the highest under CK,while RB41 is the highest under biocarbon organic fertilizer.Sphingomonas is positively correlated with sucrase activity,but negatively correlated with urease and nitrate reductase activities.Bacterial community structure under biochar organic fertilizer is significantly different from those of other treatments.In conclusion,sesame cake fertilizer and biochar organic fertilizer are beneficial to promote nitrogen mineralization in early and late stages and increase the amount of nitrogen mineralization,respectively,and the latter also has the highest the diversity and richness of soil bacteria.关键词
有机肥/植烟土壤/氮素矿化/土壤酶活性/土壤细菌群落Key words
Organic fertilizer/Tobacco-planting soil/Nitrogen mineralization/Soil enzyme activity/Soil bacterial community分类
农业科技引用本文复制引用
刘威,陈小龙,赵园园,王德勋,谢天琪,吕建国,金东峰,史宏志..有机肥施用对植烟土壤氮素矿化及土壤酶和微生物群落的影响[J].土壤,2023,60(5):1025-1034,10.基金项目
国家自然科学基金项目(42030702,42207052)、国家重点研发计划项目(2022YFD1700800)和"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2022SDZG08)资助 Supported by the National Natural Science Foundation of China(Nos.42030702 and 42207052),the National Key Research and Development Program of China(No.2022YFD1700800),and the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province,China(No.2022SDZG08) （42030702,42207052）
