土壤2023，Vol.60Issue(5)：1062-1069,8.DOI:10.13758/j.cnki.tr.2023.05.016
砂姜黑土中兼具促生功能的纤维素降解菌的筛选及其应用
Screening and Application of Cellulose Decomposing Bacteria with Growth Promoting Function on Shajiang Black Soil
摘要
Abstract
In this study,cellulose-degrading strains with plant growth promotion were isolated and screened from wheat and corn rotation system in Shajiang black soil to achieve resource utilization of straw and high-stable maize production.By monitoring carboxymethyl cellulase activity(CMC)and indoleacetic acid(IAA)secretion,a high IAA-production ability strain with excellent efficiency in cellulose breakdown was identified.The X2 strain was identified as Rhizobium pusense based on morphology and physiological and biochemical properties as well as 16S rRNA gene sequence analysis.It was found that the straw degradation ratio could reach to 16.1%on the 15 d of liquid fermentation conditions,which was 65.4%higher than that of the control;in addition,alkaline nitrogen content in the potted soil was significantly higher than that of the control after inoculation with this strain.The average root diameter,surface area,and above-ground dry weight of the plants increased significantly by 22.0%,28.6%,and 33.3%,respectively.To further improve the activity of the strain,the culture conditions of the strain were optimized using a single-factor test.The maximum growth,CMC activity,and IAA production were obtained at optimal fermentation conditions(liquid volume 25 mL/250 mL,yeast powder as nitrogen source,and pH 5.0-6.0).In summary,the sieved X2 strain has the ability of straw degradation and maize promotion,it can provide a new microbial resource for straw resource utilization in Shajiang black soil area.关键词
砂姜黑土/普沙根瘤菌/秸秆促腐/作物促生/条件优化Key words
Shajiang black soil/Rhizobium pusense/Straw decomposition/Growth promoting/Condition optimization分类
农业科技引用本文复制引用
张子赟,朱林,胡占琴,朱媛媛,卫正宇,张朝春,徐文思,马超..砂姜黑土中兼具促生功能的纤维素降解菌的筛选及其应用[J].土壤,2023,60(5):1062-1069,8.基金项目
西南大学创新研究 2035 先导计划(SWU-XDZD22001)、国家玉米产业技术体系(CARS-02-15)和中德国际合作项目(328017493/GRK 2366)资助Supported by the Innovation Research 2035 Pilot Plan of Southwest University(No.SWU-XDZD22001),National Maize Production System in China(No.CARS-02-15),and Sino-German IRTG AMAIZE-P(No.328017493/GRK 2366) （SWU-XDZD22001）
