肝素化脱细胞血管支架的制备及血液相容性评价
Preparation of heparinized acellular vascular scaffold and hemocompatibility evaluation
摘要
Abstract
BACKGROUND:Acellular vascular scaffolds can mimic the microstructure and function of native blood vessels,but some extracellular matrix loss occurs during their preparation,which affects their hemocompatibility.Therefore,it is necessary to modify them to improve their hemocompatibility. OBJECTIVE:To assess the hemocompatibility of acellular vascular scaffold prepared by Triton-x100/heparin sodium treatment. METHODS:The abdominal aorta was taken from SD rats and randomly divided into control and experimental groups.The control group was treated with Triton-x100 for 48 hours.The experimental group was treated with Triton-x100 for 48 hours and then treated with heparin sodium.The morphology and hydrophilicity of the two groups of acellular vascular scaffolds were detected.The hemocompatibility of the two groups of acellular vascular scaffold was evaluated by recalcification coagulation time test,platelet adhesion test,dynamic coagulation time test,hemolysis test,and complement activation test. RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that the surface of the two groups of vascular scaffolds was relatively intact,and a large number of fiber filaments appeared on the surface of the scaffolds after decellularity treatment,and the surface microstructure changed significantly.The water contact angle of the two groups of vascular scaffolds was smaller than that of natural vessels(P<0.000 1).There was no significant difference in water contact angle between the two groups(P>0.05).(2)The coagulation time of vascular scaffold was longer in the experimental group than in the control group(P<0.05).The number of platelets attached to the scaffold membrane was less in the experimental group than that in the control group(P<0.000 1).The coagulation index was greater in the experimental group than that in the control group(P<0.01),and the complement level was lower in the experimental group than that in the control group(P<0.001).The hemolysis rate of the two groups was lower than 5%of the national standard.(3)To conclude,acellular scaffold treated with Triton-x100/heparin sodium has excellent hemocompatibility.关键词
脱细胞血管支架/肝素化/血液相容性/心血管疾病/组织工程Key words
acellular vascular scaffold/heparinization/hemocompatibility/cardiovascular disease/tissue engineering分类
临床医学引用本文复制引用
李霞飞,赵苓苓,梁峰,张雪薇,张锦锦,林飞,杨托,赵亮..肝素化脱细胞血管支架的制备及血液相容性评价[J].中国组织工程研究,2024,28(17):2631-2636,6.基金项目
河南省自然科学基金(202300410311),项目负责人:赵亮 （202300410311）
河南省大学生创新创业训练计划项目(S202310472023),项目负责人:梁峰 （S202310472023）
新乡医学院研究生科研创新支持计划资助项目(YJSCX2022108Y),项目负责人:杨托 （YJSCX2022108Y）
新乡医学院2022年大学生科研创新课题(xyxskyz202236),项目负责人:张锦锦 （xyxskyz202236）
河南省大中专院校就业创业课题(JYB2023039),项目负责人:赵亮 Natural Science Foundation of Henan Province,No.202300410311(to ZL) （JYB2023039）
College Student Innovation and Entrepreneurship Training Program Project of Henan Province,No.S202310472023(to LF) （to LF）
Postgraduate Research and Innovation Support Plan Project of Xinxiang Medical University,No.YJSCX2022108Y(to YT) （to YT）
2022 Research and Innovation of College Students in Xinxiang Medical University,No.xyxskyz202236(to ZJJ) （to ZJJ）
Employment and Entrepreneurship Project in Colleges and Universities in Henan Province,No.JYB2023039(to ZL) （to ZL）
