中国组织工程研究2024，Vol.28Issue(17)：2667-2674,8.DOI:10.12307/2024.480
聚己内酯-聚多巴胺-AOPDM1支架在高糖环境下的促成骨性能
Polycaprolactone-polydopamine-AOPDM1 scaffold promotes bone formation in a high-glucose environment
摘要
Abstract
BACKGROUND:Oral and maxillofacial bone tissue defects can seriously affect the physical and mental health of patients.When bone defects occur in diabetic patients,bone metabolism disorders caused by abnormal blood sugar make it more difficult to repair and treat. OBJECTIVE:To attempt to apply AOPDM1,a polypeptide with potential bioactivity to the osteogenic treatment of diabetic patients. METHODS:In normal or high-glucose environment,different concentrations of AOPDM1 were used to interfere with mouse bone marrow mesenchymal stem cells,and cell proliferation,alkaline phosphatase activity,mineralization nodules formation and osteogenic differentiation gene expression were detected.The polycaprolactone scaffold was prepared by electrospinning technology,and the scaffold was modified by polydopamine to prepare the polycaprolactone-polydopamine composite scaffold.Finally,the scaffolds were placed in AOPDM1 solution to prepare polycaprolactone-polydopamine-AOPDM1 scaffolds.The water contact angle and mechanical properties of the scaffolds were tested in the three groups.In normal or high-glucose environment,the three groups of scaffolds were co-cultured with mouse bone marrow mesenchymal stem cells,respectively,and cell adhesion,alkaline phosphatase activity and osteopontin expression were detected. RESULTS AND CONCLUSION:(1)Compared with normal environment,high-glucose environment inhibited the proliferation of bone marrow mesenchymal stem cells.In the same environment,AOPDM1 could promote the proliferation of mouse bone marrow mesenchymal stem cells.When AOPDM1 concentration was the same,alkaline phosphatase activity,mineralization ability and mRNA expression of type Ⅰ collagen,osteopontin,alkaline phosphatase,and Runx2 of bone marrow mesenchymal stem cells were decreased in high-glucose environment compared with normal environment.Under the same environment,AOPDM1 could improve the alkaline phosphatase activity,mineralization ability,and mRNA expression of type Ⅰ collagen,osteopontin,alkaline phosphatase and Runx2 of bone marrow mesenchymal stem cells.(2)The hydrophilicity of polycaprolactone-polydopamine scaffold and polycaprolactone-polydopamine-AOPDM1 scaffold was higher than that of polycaprolactone scaffold(P<0.001),and there was no significant difference in tensile strength and elastic modulus among the three groups(P>0.05).Compared with the other two groups of scaffolds,the cells on the polycaprolactone-polydopamine-AOPDM1 scaffold had better adhesion morphology.When the scaffolds were identical,compared with normal environment,high-glucose environment inhibited alkaline phosphatase activity and osteopontin expression of bone marrow mesenchymal stem cells.When the environment was the same,the alkaline phosphatase activity and osteopontin expression of bone marrow mesenchymal stem cells on the polycaprolactone-polydopamine-AOPDM1 scaffold were higher than those on the other two scaffolds.(3)The above results prove that polycaprolactone-polydopamine-AOPDM composite scaffold can promote the osteogenic properties of bone marrow mesenchymal stem cells in high-glucose environment.关键词
多肽/聚己内酯/聚多巴胺/复合支架/骨髓间充质干细胞/高糖微环境/细胞增殖/成骨分化Key words
polypeptide/polycaprolactone/polydopamine/composite scaffold/bone marrow mesenchymal stem cell/high-glucose microenvironment/cell proliferation/osteogenic differentiation分类
医药卫生引用本文复制引用
刘子杨,劳安,徐陈词,AIRI SHIN,吴嘉晴,刘加强..聚己内酯-聚多巴胺-AOPDM1支架在高糖环境下的促成骨性能[J].中国组织工程研究,2024,28(17):2667-2674,8.基金项目
国家自然科学基金面上项目(82071081),项目名称:PDGF-BB/PI3K信号轴在高糖引发GSK-3β介导的成骨抑制过程中的调控机理,项目负责人:刘加强 （82071081）
上海市卫生健康委员会卫生行业临床研究专项(卓越项目)(20224Z0010),项目名称:微创颌骨手术联合正畸治疗改善轻度骨性上颌前突的临床研究,项目负责人:刘加强 National Natural Science Foundation of China(General Program),No.82071081(to LJQ) （卓越项目）
Shanghai Municipal Health Commission Health Industry Clinical Research Project(Excellence Project),No.20224Z0010(to LJQ) （Excellence Project）
