中国组织工程研究2024，Vol.28Issue(17)：2696-2701,6.DOI:10.12307/2024.410
成骨诱导因子缓释系统修饰国产多孔钽对MG63细胞功能的影响
Effect of domestic porous tantalum modified by osteogenic induction factor slow-release system on function of MG63 cells
摘要
Abstract
BACKGROUND:Previous research by the research team found that domestically produced porous tantalum is beneficial for early adhesion and proliferation of MG63 cells,and can be used as a scaffold material for bone tissue engineering. OBJECTIVE:To investigate the effect of domestic porous tantalum modified by osteogenic induction factor slow-release system on the adhesion,proliferation,and differentiation of MG63 cells. METHODS:Osteogenic induction factor slow-release system was constructed by adding 15%volume fraction of osteogenic factor solution to poly(lactic-co-glycolic-acid)gel.The passage 3 MG63 cells were inoculated on a porous tantalum surface(control group),porous tantalum surface coated with poly(lactic-co-glycolic-acid)copolymer gel(gel group),and porous tantalum surface coated with osteoblastic induction factor slow-release system(slow-release system group),and co-cultured for 5 days.The surface cytoskeleton of the material was observed by phalloidine staining.Cell proliferation was detected by flow cytometry.Western blot assay and RT-qPCR were used to detect the protein and mRNA expressions of type Ⅰ collagen,osteopontin,and RUNX-2 on the surface cells of the material. RESULTS AND CONCLUSION:(1)Phalloidine staining showed that MG63 cells adhered to and grew on the surface and inside of the three groups of porous tantalum,and the matrix secreted by the cells covered the surface of the material.(2)Flow cytometry showed that the cell proliferation in the slow-release system group was faster than that in the control group and the gel group(P<0.05).(3)Western blot assay and RT-qPCR showed that the protein and mRNA expressions of type Ⅰ collagen,osteopontin,and RUNX-2 in the slow-release system group were higher than those in the control group and gel group(P<0.05).(4)The results showed that the domestic porous tantalum modified by the osteogenic induction factor slow-release system was beneficial to the adhesion,proliferation,and differentiation of MG63 osteoblasts.关键词
多孔钽/MG63细胞/缓释系统/成骨诱导因子/聚乳酸-羟基乙酸共聚化合物Key words
porous tantalum/MG63 cell/slow-release system/osteogenic induction factor/poly(lactic-co-glycolic-acid)copolymer分类
临床医学引用本文复制引用
郭小玲,李粤源,徐田杰,张辉,王志强,王茜..成骨诱导因子缓释系统修饰国产多孔钽对MG63细胞功能的影响[J].中国组织工程研究,2024,28(17):2696-2701,6.基金项目
国家科技支撑计划课题资助项目(2012BAE06B03),项目负责人:王志强 （2012BAE06B03）
河北省科技支撑资助项目(16277776D),项目负责人:王志强 （16277776D）
三三人才工程(C20221117),项目负责人:张辉 （C20221117）
河北省 2023 年度医学科学研究课题(20230215),项目负责人:王茜 National Science and Technology Support Program,No.2012BAE06B03(to WZQ) （20230215）
Hebei Province Science and Technology Support Project,No.16277776D(to WZQ) （to WZQ）
Three-Three Talent Project,No.C20221117(to ZH) （to ZH）
2023 Medical Science Research Project in Hebei Province,No.20230215(to WQ) （to WQ）
