心肌补片:细胞来源、完善策略及最佳制作方法分析
Myocardial patch:cell sources,improvement strategies,and optimal production methods
摘要
Abstract
BACKGROUND:Myocardial patches are used as an effective way to repair damaged myocardium,and there is controversy over which cells to use to make myocardial patches and how to maximize the therapeutic effect of myocardial patches in vivo. OBJECTIVE:To find out the best way to make myocardial patches by overviewing the cellular sources of myocardial patches and strategies for perfecting them. METHODS:The first author searched PubMed and Web of Science databases by using"cell sheet,cell patch,cardiomyocytes,cardiac progenitor cells,fibroblasts,embryonic stem cell,mesenchymal stem cells"as English search terms,and searched CNKI and Wanfang databases by using"myocardial patch,biological 3D printing,myocardial"as Chinese search terms.After enrollment screening,94 articles were ultimately included in the result analysis. RESULTS AND CONCLUSION:(1)The cellular sources of myocardial patches are mainly divided into three categories:somatic cells,monoenergetic stem cells,and pluripotent stem cells,respectively.There are rich sources of cells for myocardial patches,but not all of them are suitable for making myocardial patches,e.g.,myocardial patches made from fibroblasts and skeletal myoblasts carry a risk of arrhythmogenicity,and mesenchymal stem cells have a short in vivo duration of action and ethical concerns.With the discovery of induced multifunctional stem cells,a reliable source of cells for making myocardial patches is available.(2)There are two methods of making myocardial patches.One is using cell sheet technology.The other is using biological 3D printing technology.Cell sheet technology can preserve the extracellular matrix components intact and can maximally mimic the cell growth ring in vivo.However,it is still difficult to obtain myocardial patches with three-dimensional structure by cell sheet technology.Biologicasl 3D printing technology,however,can be used to obtain myocardial patches with three-dimensional structures through computerized personalized design.(3)The strategies for perfecting myocardial patches mainly include:making myocardial patches after co-cultivation of multiple cells,improving the ink formulation and scaffold composition in biological 3D printing technology,improving the therapeutic effect of myocardial patches,suppressing immune rejection after transplantation,and perfecting the differentiation and cultivation protocols of stem cells.(4)There is no optimal cell source or method for making myocardial patches,and myocardial patches obtained from a particular cell or technique alone often do not achieve the desired therapeutic effect.Therefore,researchers need to choose the appropriate strategy for making myocardial patches based on the desired therapeutic effect before making them.关键词
心肌补片/多能干细胞/3D打印/生物墨水/细胞片/综述/间充质干细胞/免疫反应/心肌细胞/骨骼肌母细胞Key words
myocardial patch/pluripotent stem cell/3D printing/bioink/cell sheet/review/mesenchymal stem cell/immune response/cardiomyocyte/skeletal myoblast分类
医药卫生引用本文复制引用
胡威,邢健,陈广新,陈泽娥,赵艺,乔丹,欧阳昆富,黄文华..心肌补片:细胞来源、完善策略及最佳制作方法分析[J].中国组织工程研究,2024,28(17):2723-2730,8.基金项目
国家重点研发计划(2022YFB4600600),项目负责人:黄文华 （2022YFB4600600）
国家自然科学基金(31972915,32271181),项目负责人:黄文华 （31972915,32271181）
国家自然科学基金面上项目(81970421,82170235),项目负责人:欧阳昆富 （81970421,82170235）
中国博士后科学基金(2021M702224,2022T150430),项目负责人:陈泽娥 （2021M702224,2022T150430）
广东省基础与应用基础研究基金(2020B1515120001),项目负责人:黄文华 （2020B1515120001）
广东省自然科学基金面上项目(2023A1515011842),项目负责人:欧阳昆富 （2023A1515011842）
广东省基础与应用基础研究基金项目(2019A1515111178),项目负责人:赵艺 （2019A1515111178）
牡丹江医学院科学基金火炬计划(2022-MYHJ-004),项目负责人:邢健 （2022-MYHJ-004）
深圳市自然科学基金(JCYJ20190808174001746,JCYJ20210324105407019),项目负责人:欧阳昆富 （JCYJ20190808174001746,JCYJ20210324105407019）
深港脑科学创新研究院项目(2019SHIBS0004,2023SHIBS0004),项目负责人:欧阳昆富 National Key Research&Development Program,No.2022YFB4600600(to HWH) （2019SHIBS0004,2023SHIBS0004）
National Natural Science Foundation of China,No.31972915,32271181(to HWH) （to HWH）
General Project of National Natural Science Foundation of China,No.81970421,82170235(to OYKF) （to OYKF）
China Postdoctoral Science Foundation,No.2021M702224,2022T150430(to CZE) （to CZE）
Guangdong Basic and Applied Basic Research Fund,No.2020B1515120001(to HWH) （to HWH）
General Project of Guangdong Natural Science Foundation,No.2023A1515011842(to OYKF) （to OYKF）
Guangdong Basic and Applied Basic Research Fund Project,No.2019A1515111178(to ZY) （to ZY）
Science Foundation Torch Program of Mudanjiang Medical University,No.2022-MYHJ-004(to XJ) （to XJ）
Shenzhen Natural Science Foundation,No.JCYJ20190808174001746,JCYJ20210324105407019(to OYKF) （to OYKF）
Shenzhen-Hong Kong Brain Science Innovation Institute Project,No.2019SHIBS0004,2023SHIBS0004(to OYKF) （to OYKF）
