现代医药卫生2023，Vol.39Issue(23)：3966-3969,3976,5.DOI:10.3969/j.issn.1009-5519.2023.23.002
PLIN2在氧化型低密度脂蛋白诱导的泡沫细胞脂噬中的作用
The role of PLIN2 in oxidized low density lipoprotein-induced foam cell lipophagy
摘要
Abstract
Objective Foam cell formation was an important part of the occurrence and development of atherosclerosis.To explore the role of perilipin2(PLIN2)in foam cell lipophagy,and to provide more ideas for the prevention and treatment of atherosclerosis-related diseases.Methods The foam cell model induced by ox-idized low density lipoprotein(ox-LDL)was constructed,and the expression of PLIN2 gene in the cells was si-lenced.The cells were divided into five groups:the control group,the empty plasmid group,the ox-LDL treat-ment group,the siPLIN2 group,the ox-LDL+siPLIN2 group.The oil red O staining was used to observe the lipid accumulation of intracellular,and the Western blot was used to observe the expression of PLIN2 and lipo-phagy-related proteins LC3 and p62.Results During the formation of foam cells induced by ox-LDL,the ex-pression of LC3 increased with the increase of lipid,while p62 decreased first and then increased.The expres-sion of LC3 was down-regulated in PLIN2-silenced RAW264.7 macrophages.In PLIN2-silenced foam cells,the expression of p62 protein was significantly increased and lipophagy was weakened.Conclusion In ox-LDL-induced foam cells,knockdown of PLIN2 can down-regulate the expression of p62 and attenuate intracel-lular lipophagy.关键词
脂噬/围脂滴蛋白2/氧化型低密度脂蛋白/泡沫细胞Key words
Lipophagy/Perilipin2/Oxidized low density lipoprotein/Foam cells分类
医药卫生引用本文复制引用
谭艳美,陈三俊,谭玉林,吴智,蒙国照,谭艳飞..PLIN2在氧化型低密度脂蛋白诱导的泡沫细胞脂噬中的作用[J].现代医药卫生,2023,39(23):3966-3969,3976,5.基金项目
湖南省教育厅科学研究项目(20C1701) （20C1701）
湖南省卫生健康委员会立项课题(D202303067556) （D202303067556）
湘南学院校级科学研究项目(2020XJ39). （2020XJ39）
